Moulari Brice, Lboutounne Hassan, Chaumont Jean-Pierre, Guillaume Yves, Millet Joëlle, Pellequer Yann
Equipe Sciences Séparatives et Biopharmaceutiques UFR Sciences Médicales et Pharmaceutiques, Université de Franche-Comté, EA-3924, Place Saint Jacques, Besançon, France.
Acta Odontol Scand. 2006 Jun;64(3):153-8. doi: 10.1080/00016350500483152.
Harungana madagascariensis Lam. ex Poir. (Hypericaceae) is known to have biological properties with mainly antibacterial, antifungal, and antiviral effects. The objective of this study was to investigate the in vitro bactericidal activity of the ethyl acetate H. madagascariensis leaf extract (HLE) on the main oral bacterial strains largely implicated in dental caries and gingivitis infections, and the possibility of potentialization of HLE antibacterial effects using the poly (D,L-lactide-co-glycolide) nanoparticles (PLG-NP). The microdilution technique and the interfacial polymer deposition following the solvent diffusion method were used to investigate the in vitro bactericidal activity of ethyl acetate HLE and to prepare nanoparticles, respectively. HLE showed significant bactericidal effects against the bacterial strains tested, with minimal bactericidal concentration (MBC) to 5 x 10(2) mg/l or less, except for Lactobacillus casei with 7.5 x 10(2) mg/l. With the HLE incorporated into PLG nanoparticles (HLE-PLG-NP), we observed diminution of the bactericidal concentration compared to HLE, the upper MBC being of 1.875 x 10(2) mg/l. Incorporation of the HLE into a colloidal carrier optimized its antibacterial performance.
马达加斯加金丝桃（Harungana madagascariensis Lam. ex Poir.，金丝桃科）已知具有主要抗菌、抗真菌和抗病毒作用的生物学特性。本研究的目的是调查马达加斯加金丝桃叶乙酸乙酯提取物（HLE）对主要引发龋齿和牙龈炎感染的口腔细菌菌株的体外杀菌活性，以及使用聚（D，L-丙交酯-共-乙交酯）纳米颗粒（PLG-NP）增强HLE抗菌效果的可能性。分别采用微量稀释技术和溶剂扩散法后的界面聚合物沉积法来研究乙酸乙酯HLE的体外杀菌活性和制备纳米颗粒。HLE对所测试的细菌菌株显示出显著的杀菌作用，除干酪乳杆菌的最低杀菌浓度（MBC）为7.5×10²mg/l外，其他菌株的MBC为5×10²mg/l或更低。将HLE掺入PLG纳米颗粒（HLE-PLG-NP）后，与HLE相比，我们观察到杀菌浓度降低，最高MBC为1.875×10²mg/l。将HLE掺入胶体载体中优化了其抗菌性能。
