Biapa Prosper-Cabral N, Agbor Gabriel A, Oben Julius E, Ngogang Jeanne Y
Department of Biochemistry, University of Yaoundé I, P.O Box 812, Yaoundé, Cameroon.
Afr J Tradit Complement Altern Med. 2007 Jun 10;4(4):495-500. doi: 10.4314/ajtcam.v4i4.31243.
Four plants (Amphimas pterocaroides, Harungana madagascariensis, Myrianthus arboreus, and Cussonia barteri) that are commonly used in Cameroon for the management or reversal of anaemia were screened for their phytochemical content and antioxidant potential. Four extracts (methanolic, hydro-ethanolic, aqueous, and hydrolysed) from each of the plants were prepared and analysed. Qualitative phytochemical tests were used to detect the presence of alkaloids, tannins, saponins, flavonoids, glycosides and phenols, while three quantitative methods; Folin, Ferric Reducing Antioxidant Power (FRAP) and diphenyl-1, 2-picryl hydrazyl (DPPH) were used to determine the antioxidant potential of these extracts. With the exception of the extracts of Cussonia barteri (negative for triterpenes and phenols) and the aqueous extract of Harungana madagascariensis (negative result for cardiac glycosides and glycosides), all other extracts contained the phytochemicals tested. The highest antioxidant activities were observed in the hydrolysed extracts of each plant, while the aqueous extract showed the least activity irrespective of the method used. The presence of active phytochemical substances with antioxidant activities may provide substantial basis for the use of these plants in ethnomedicine.
对喀麦隆常用于治疗或逆转贫血的四种植物（翼豆木、马达加斯加哈伦加那、大果刺篱木和巴氏刺参）进行了植物化学成分和抗氧化潜力的筛选。制备并分析了每种植物的四种提取物（甲醇提取物、氢乙醇提取物、水提取物和水解提取物）。采用定性植物化学试验检测生物碱、单宁、皂苷、黄酮类化合物、糖苷和酚类的存在，同时采用三种定量方法：福林法、铁还原抗氧化能力（FRAP）法和二苯基-1,2-苦基肼（DPPH）法测定这些提取物的抗氧化潜力。除巴氏刺参提取物（三萜和酚类呈阴性）和马达加斯加哈伦加那水提取物（强心苷和糖苷呈阴性结果）外，所有其他提取物均含有所检测的植物化学成分。每种植物的水解提取物抗氧化活性最高，而水提取物无论采用何种方法活性都最低。具有抗氧化活性的活性植物化学物质的存在可能为这些植物在民族医学中的应用提供坚实的基础。
