Moura F E A, Ribeiro D C S, Gurgel N, da Silva Mendes A C, Tavares F N, Timóteo C N G, da Silva E E
Laboratory of Virology, Departamento de Patologia e Medicina Legal, Universidade Federal do Ceará, Rua Monsenhor Furtado SN, Rodolfo Teófilo, 60 441-750, Fortaleza-Ceará, Brazil.
Br J Ophthalmol. 2006 Sep;90(9):1091-3. doi: 10.1136/bjo.2006.098822. Epub 2006 Jun 29.
BACKGROUND/AIM: Between February and May 2003 an epidemic of acute haemorrhagic conjunctivitis affected more than 200 000 people in all five geographic regions of Brazil (north, south, midwestern, southeast, and northeast). The aim was to identify the aetiological agent and to describe clinical aspects of this outbreak in a group of patients treated at the ophthalmology department of the Hospital Walter Cantídio (OD-HWC) at the Universidade Federal do Ceará, in the city of Fortaleza, capital of the state of Ceará, northeastern Brazil.
Conjunctival swabs were collected from patients who spontaneously went to the laboratory of virology. Specimens were inoculated in HEp-2 and RD cell lines. The viral isolation was confirmed by performing reverse transcriptase polymerase chain reaction and indirect immunofluorescence assay.
Viral conjunctivitis was diagnosed in 56 patients but only 24 of them allowed the collection of samples. Of 24 conjunctival swabs tested, 11 were positive for a variant of coxsackie virus A24 (CA24v) and one of the isolates reacted with anti-adenovirus monoclonal antibodies.
CA24v was confirmed as the aetiological agent of this outbreak of acute haemorrhagic conjunctivitis in the city of Fortaleza.
背景/目的：2003年2月至5月期间，巴西所有五个地理区域（北部、南部、中西部、东南部和东北部）有超过20万人感染急性出血性结膜炎。目的是确定病原体，并描述在巴西东北部塞阿拉州首府福塔莱萨市的塞阿拉联邦大学沃尔特·坎蒂迪奥医院眼科（OD-HWC）接受治疗的一组患者中此次疫情的临床特征。
从自行前往病毒学实验室的患者中采集结膜拭子。将标本接种于HEp-2和RD细胞系。通过逆转录聚合酶链反应和间接免疫荧光试验确认病毒分离。
56例患者被诊断为病毒性结膜炎，但其中只有24例采集了样本。在检测的24份结膜拭子中，11份对柯萨奇病毒A24变种（CA24v）呈阳性，其中一份分离株与抗腺病毒单克隆抗体发生反应。
CA24v被确认为福塔莱萨市此次急性出血性结膜炎疫情的病原体。
