对人类组蛋白H2AZ在体内沉积的分析表明，它在表观遗传模板机制中不发挥直接作用。
Analysis of human histone H2AZ deposition in vivo argues against its direct role in epigenetic templating mechanisms.
作者信息
Viens Antoine, Mechold Undine, Brouillard Franck, Gilbert Cristele, Leclerc Philippe, Ogryzko Vasily
机构信息
CNRS UMR 8126, Institut Gustave-Roussy, PR1, 39 rue Camille Desmoulin, 94100 Villejuif, France.
出版信息
Mol Cell Biol. 2006 Jul;26(14):5325-35. doi: 10.1128/MCB.00584-06.
Abstract
Chromatin is considered to be a principal carrier of epigenetic information due to the ability of alternative chromatin states to persist through generations of cell divisions and to spread on DNA. Replacement histone variants are novel candidates for epigenetic marking of chromatin. We developed a novel approach to analyze the chromatin environment of nucleosomes containing a particular replacement histone. We applied it to human H2AZ, one of the most studied alternative histones. We find that neither H2AZ itself nor other features of the H2AZ-containing nucleosome spread to the neighboring nucleosomes in vivo, arguing against a role for H2AZ as a self-perpetuating epigenetic mark.
摘要
由于染色质的不同状态能够在细胞分裂的世代中持续存在并在DNA上扩散，因此染色质被认为是表观遗传信息的主要载体。替换组蛋白变体是染色质表观遗传标记的新候选者。我们开发了一种新方法来分析含有特定替换组蛋白的核小体的染色质环境。我们将其应用于人类H2AZ，这是研究最多的替代组蛋白之一。我们发现，无论是H2AZ本身还是含H2AZ的核小体的其他特征，在体内都不会扩散到相邻的核小体，这表明H2AZ不具有作为自我延续的表观遗传标记的作用。
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