Yang Y S, Lee T Y
Department of Obstetrics and Gynecology, National Taiwan University Hospital, Taipei, R.O.C.
J Formos Med Assoc. 1991 May;90(5):514-6.
In this study, we determined whether there was a correlation between sperm penetration assay (SPA) and acrosome reaction (AR) in sperm after being treated with human follicular fluid (hFF). Sperm specimens were obtained from 13 men participating in our in vitro fertilization program. After washing and preincubation, aliquots of sperm samples were treated with either hFF or Ham's F-10 medium (control). Approximately 1.0 x 10(6) motile sperm from the control and hFF-treated samples were coincubated individually with zona-free hamster eggs for SPA. Sperm remaining in the suspension after coincubation with eggs were taken to determine the incidence of AR by a triple-stain technique. The data revealed that an increased SPA score in hFF-treated samples was related to a higher incidence of acrosome-reacted sperm.
在本研究中，我们确定了经人卵泡液（hFF）处理后的精子，其精子穿透试验（SPA）与顶体反应（AR）之间是否存在相关性。精子标本取自参与我们体外受精项目的13名男性。洗涤并预孵育后，将精子样本的等分试样分别用hFF或Ham's F - 10培养基（对照）处理。将来自对照和hFF处理样本的约1.0×10⁶条活动精子分别与去透明带仓鼠卵共同孵育以进行SPA。与卵共同孵育后留在悬浮液中的精子用于通过三重染色技术确定AR的发生率。数据显示，hFF处理样本中SPA评分的增加与顶体反应精子的较高发生率相关。
