Siegel M S, Paulson R J, Graczykowski J W
University of Southern California School of Medicine, Department of Obstetrics and Gynecology, Los Angeles 90033.
Hum Reprod. 1990 Nov;5(8):975-80. doi: 10.1093/oxfordjournals.humrep.a137231.
The present study was designed to assess physiological and enzymatic changes in human spermatozoa following incubation in human follicular fluid (HFF). Initially, it was determined that infertility patients (n = 29) scored dramatically higher on the hamster egg penetration test (HEPT) when spermatozoa were incubated with HFF (22.9 +/- 4.4%) rather than the standard swim-up alone (4.6 +/- 1.1%). To further evaluate this effect, in experiment I, spermatozoa were obtained from proven fertile individuals and evaluated following exposure to three different HFF samples as well as control treatments (medium, cul de sac fluid and heparin). There were no significant differences in HEPT scores following sperm incubation in the three different follicular fluids although incubation in all the fluids significantly (P less than 0.01) enhanced sperm penetration (%PEN) when compared to the standard swim-up and other control treatments. The absence of an effect of cul de sac fluid on spermatozoa indicates that not all body fluids contain factors which stimulate sperm fertilizing capacity. The effect of HFF was demonstrated in a infertile patient population as well as in donors of proven fertility. In experiment II, the effect of HFF on the acrosome reaction (%AR), sperm fertilizing capacity and changes in sperm proteolytic enzymes were determined. There was no significant difference in the %AR between freshly ejaculated (7.9 +/- 3.1) and medium incubated (9.4 +/- 1.6) spermatozoa; however, in both of these treatments the %AR was less (P less than 0.01) than for spermatozoa treated with HFF (45.6 +/- 4.7). The %PEN following incubation of spermatozoa in HFF (52.2 +/- 6.8) was greatly increased (P +/- 0.01) compared to the standard swim-up (19 +/- 3.9).
本研究旨在评估人类精子在人卵泡液（HFF）中孵育后的生理和酶变化。最初，研究发现，与仅采用标准上游法（4.6±1.1%）相比，不育患者（n = 29）的精子与人卵泡液一起孵育时，在仓鼠卵穿透试验（HEPT）中的得分显著更高（22.9±4.4%）。为了进一步评估这种效应，在实验I中，从经证实有生育能力的个体获取精子，并在暴露于三种不同的人卵泡液样本以及对照处理（培养基、盆腔积液和肝素）后进行评估。精子在三种不同卵泡液中孵育后的HEPT得分没有显著差异，尽管与标准上游法和其他对照处理相比，在所有这些液体中孵育均显著（P<0.01）提高了精子穿透率（%PEN）。盆腔积液对精子无影响，这表明并非所有体液都含有刺激精子受精能力的因子。人卵泡液的作用在不育患者群体以及经证实有生育能力的供体中均得到了证明。在实验II中，测定了人卵泡液对顶体反应（%AR）、精子受精能力和精子蛋白水解酶变化的影响。新鲜射出的精子（7.9±3.1）和在培养基中孵育的精子（9.4±1.6）之间的%AR没有显著差异；然而，在这两种处理中，%AR均低于用人卵泡液处理的精子（45.6±4.7）（P<0.01）。与标准上游法（19±3.9）相比，精子在人卵泡液中孵育后的%PEN（52.2±6.8）大幅提高（P<0.01）。
