Triepel J, Grimmelikhuijzen C J
Histochemistry. 1984;80(1):63-71. doi: 10.1007/BF00492773.
Several reports (cf. Weber et al. (1981) Science 214:1248-1251) have described the extensive occurrence, in rat brain, of material immunologically related to the molluscan neuropeptide FMRFamide. We have reexamined these data in guinea pig and rat, using six different antisera to FMRFamide. Immunoreactive perikarya and fibres were found to be distributed throughout the rodent brain (Table 1). This distribution was roughly similar to that found by Weber and coworkers. However, solid-phase absorption of the antisera with bovine pancreatic polypeptide, which shares an arginine and an amidated aromatic amino acid (RYamide) with FMRFamide, showed that staining in most regions could be due to crossreactivity with bovine pancreatic polypeptide-like (or neuropeptide Y-like) material. Double-labelling experiments with antisera to FMRFamide and bovine pancreatic polypeptide led to the same conclusion. The only structures where no apparent crossreactivity occurred were perikarya and fibres in the nucleus dorsomedialis, ventromedialis, periventricularis and paraventricularis hypothalami and fibres in the area lateralis hypothalami, nucleus parabrachialis, substantia grisea centralis mesencephali, various parts of the formatio reticularis, and spinal cord. Hence only these structures might contain material which is more related to the molluscan tetrapeptide.
几份报告（参见Weber等人（1981年）《科学》214:1248 - 1251）描述了在大鼠脑中广泛存在的、与软体动物神经肽FMRF酰胺免疫相关的物质。我们使用六种不同的抗FMRF酰胺血清，在豚鼠和大鼠中重新检查了这些数据。发现免疫反应性的胞体和纤维分布于整个啮齿动物脑内（表1）。这种分布与Weber及其同事所发现的大致相似。然而，用牛胰多肽对血清进行固相吸收，牛胰多肽与FMRF酰胺共有一个精氨酸和一个酰胺化芳香氨基酸（RY酰胺），结果表明大多数区域的染色可能是由于与牛胰多肽样（或神经肽Y样）物质的交叉反应。用抗FMRF酰胺和牛胰多肽的血清进行双重标记实验也得出了相同的结论。唯一未出现明显交叉反应的结构是下丘脑背内侧核、腹内侧核、室周核和室旁核中的胞体和纤维，下丘脑外侧区的纤维，臂旁核，中脑中央灰质，网状结构的各个部分，以及脊髓。因此，只有这些结构可能含有与软体动物四肽更相关的物质。
