DeAngelis R L, Simmons W S, Nichol C A
J Chromatogr. 1975 Mar 19;106(1):41-9. doi: 10.1016/s0021-9673(01)81045-1.
Several 2,4-diaminopyrimidines which inhibit the enzyme dihydrofolate reductase are quantitated following extraction and separation on silica gel thin-layer chromatographic plates. These compounds are candidates for the treatment of brain tumors and meningeal leukemia, because they have the ability to cross the blood-brain barrier. The ultraviolet absorption of the pyrimidine ring at 275 nm is utilized to quantitate these compounds on thin-layer chromatographic plates with a scanning instrument. This method offers the advantages of speed, specificity, versatility and sensitivity, and has proven to be satisfactory for the measurement of as little as 10 ng/ml of these compounds in biological fluids.
几种抑制二氢叶酸还原酶的2,4-二氨基嘧啶在硅胶薄层色谱板上进行萃取和分离后进行定量分析。这些化合物有望用于治疗脑肿瘤和脑膜白血病，因为它们能够穿越血脑屏障。利用嘧啶环在275nm处的紫外吸收，通过扫描仪器在薄层色谱板上对这些化合物进行定量。该方法具有快速、特异、通用和灵敏等优点，已证明可用于测定生物流体中低至10 ng/ml的这些化合物，效果令人满意。
