Filipová M, Bujdákova H, Drahovská H, Lisková A, Hanzen J
Department of Microbiology and Virology, Faculty of Science, Comenius University, Bratislava, Slovakia.
Folia Microbiol (Praha). 2006;51(1):57-61. doi: 10.1007/BF02931451.
The genes coding for 4 aminoglycoside-modifying enzymes AAC(6')-APH(2"), APH(3'), ANT(4') and ANT(6) were determined in 44 Slovak clinical isolates of Enterococcus faecalis with high-level resistance to gentamicin (HLGR, collection 1) and 48 E. faecalis isolates with resistance to amikacin (AR, collection 2). The occurrence of spotted genes was (collection 1 vs. collection 2): aac(6)-aph(2") 81.8 vs. 8.3 %, ant(4') 52.3 vs. 81.3 %, aph(3') 50 vs. 56.3 % and ant(6) 6.8 vs. 4.2 %, the most frequent combinations of genes in the HLGR collection were aac(6')-aph(2") + ant(4') and aac(6')-aph(2") + aph(3). In contrast, the aph(3') + ant(4') gene profile was predominant in AR isolates. None of the isolates contained all four AGME genes simultaneously.
在44株对庆大霉素具有高水平耐药性的斯洛伐克临床分离粪肠球菌（HLGR，集合1）和48株对阿米卡星耐药的粪肠球菌分离株（AR，集合2）中，测定了编码4种氨基糖苷类修饰酶AAC(6')-APH(2")、APH(3')、ANT(4')和ANT(6)的基因。斑点基因的出现情况为（集合1与集合2相比）：aac(6)-aph(2")分别为81.8%和8.3%，ant(4')分别为52.3%和81.3%，aph(3')分别为50%和56.3%，ant(6)分别为6.8%和4.2%。HLGR集合中最常见的基因组合是aac(6')-aph(2") + ant(4')和aac(6')-aph(2") + aph(3)。相比之下，aph(3') + ant(4')基因谱在AR分离株中占主导地位。没有分离株同时包含所有四种AGME基因。
