垂体腺苷酸环化酶激活多肽在绿头鸭初级淋巴器官中的表达
Expression of pituitary adenylate cyclase-activating polypeptide in the primary lymphoid organs of the duck Anas platyrhynchos.
作者信息
Squillacioti Caterina, Mirabella Nicola, De Luca Adriana, Paino Giuseppe
机构信息
Department of Structure, Functions and Biological Technologies, University of Naples Federico II, Italy.
出版信息
J Anat. 2006 Jul;209(1):51-8. doi: 10.1111/j.1469-7580.2006.00592.x.
The expression of pituitary adenylate cyclase-activating polypeptide (PACAP) was studied in the thymus and bursa of Fabricius of the duck Anas platyrhynchos, at different ages, using immunohistochemistry, Western blotting, RT-PCR and sequencing. In the thymus, PACAP immunoreactivity (-ir) was found in lymphoid cells. CD68/ and PGP 9.5/PACAP38 double labelling showed that PACAP was not expressed either in macrophages or in epithelial cells, suggesting that the PACAP-positive cells observed were lymphoid cells. Immunoreactive lymphocytes were observed in the interlobular septa. They increased in number with ageing. In the bursa, PACAP-ir was found in nerve fibres and in a few lymphoid cells. RT-PCR revealed PACAP mRNA expression in the thymus but not in the bursa. These results suggest that PACAP plays a role in the functions of the immune system in birds.
运用免疫组织化学、蛋白质免疫印迹法、逆转录-聚合酶链反应（RT-PCR）及测序技术，研究了不同年龄的鸭（绿头鸭）胸腺和法氏囊中垂体腺苷酸环化酶激活多肽（PACAP）的表达情况。在胸腺中，淋巴样细胞存在PACAP免疫反应性（-ir）。CD68与PGP 9.5/PACAP38双重标记显示，巨噬细胞和上皮细胞中均未表达PACAP，这表明观察到的PACAP阳性细胞为淋巴样细胞。在小叶间隔中观察到免疫反应性淋巴细胞，其数量随年龄增长而增加。在法氏囊中，神经纤维和少数淋巴样细胞存在PACAP-ir。RT-PCR显示胸腺中有PACAP mRNA表达，而法氏囊中未检测到。这些结果表明，PACAP在鸟类免疫系统功能中发挥作用。
Zotero 插件