Kerneis S, Bilge S S, Fourel V, Chauviere G, Coconnier M H, Servin A L
Département de Microbiologie et Immunologie, UFR Sciences Pharmaceutiques Paris XI, Châtenay-Malabry, France.
Infect Immun. 1991 Nov;59(11):4013-8. doi: 10.1128/iai.59.11.4013-4018.1991.
Whole diffusely adhering Escherichia coli (DAEC) C1845 cells bearing the F1845 adhesive factor bind diffusely to differentiated human colon carcinoma cell lines HT-29 and Caco-2. By using antibodies directed against the purified fimbrial adhesin F1845 factor, the expression of the DAEC F1845-specific brush border receptors in the polarized human intestinal HT-29 and Caco-2 epithelial cells was studied by indirect immunofluorescence. A low level of DAEC F1845 receptors in undifferentiated intestinal cells was detected; they were localized in a cluster of cells. DAEC F1845 receptors were expressed at a high level in differentiated HT-29 and Caco-2 cells. DAEC F1845 receptors were expressed at a strikingly high level in the apical domains of the cells and developed during enterocytic differentiation in culture, in parallel with the apical expression of the intestinal brush border hydrolase, sucrase-isomaltase.
携带F1845黏附因子的全弥漫性黏附大肠杆菌（DAEC）C1845细胞可弥漫性地黏附于分化的人结肠癌细胞系HT - 29和Caco - 2。通过使用针对纯化菌毛黏附素F1845因子的抗体，采用间接免疫荧光法研究了DAEC F1845特异性刷状缘受体在极化的人肠道HT - 29和Caco - 2上皮细胞中的表达情况。在未分化的肠道细胞中检测到低水平的DAEC F1845受体；它们定位于一群细胞中。DAEC F1845受体在分化的HT - 29和Caco - 2细胞中高水平表达。DAEC F1845受体在细胞的顶端区域显著高水平表达，并在培养过程中的肠细胞分化过程中形成，与肠道刷状缘水解酶蔗糖酶 - 异麦芽糖酶的顶端表达平行。
