Kikuta L C, Puolakkainen M, Kuo C C, Campbell L A
Department of Pathobiology, University of Washington, Seattle 98195.
Infect Immun. 1991 Dec;59(12):4665-9. doi: 10.1128/iai.59.12.4665-4669.1991.
Chlamydia pneumoniae has emerged as an important human respiratory pathogen. From a lambda gt11 gene bank constructed from C. pneumoniae isolate AR-39 DNA, an immunoreactive plaque containing a 3.0-kb insert was purified. In immunoblots, a 60-kDa protein was recognized by anti-C. pneumoniae rabbit immune serum. The recombinant protein was reactive with a Chlamydia genus-specific monoclonal antibody recognizing a 60-kDa protein found in the Sarkosyl-soluble fraction and with rabbit immune serum prepared against the Chlamydia trachomatis 60-kDa GroEL homolog associated with the delayed-type hypersensitivity response. DNA sequence analysis confirmed that the C. pneumoniae gene product is an analog of the C. trachomatis delayed-type hypersensitivity antigen and the Escherichia coli GroEL heat shock protein.
肺炎衣原体已成为一种重要的人类呼吸道病原体。从由肺炎衣原体分离株AR-39 DNA构建的λgt11基因文库中，纯化出一个含有3.0 kb插入片段的免疫反应性噬菌斑。在免疫印迹中，一种60 kDa的蛋白质被抗肺炎衣原体兔免疫血清识别。该重组蛋白与一种衣原体属特异性单克隆抗体发生反应，该抗体识别在 Sarkosyl 可溶部分中发现的一种60 kDa的蛋白质，并且与针对沙眼衣原体60 kDa GroEL同源物制备的兔免疫血清发生反应，该同源物与迟发型超敏反应相关。DNA序列分析证实，肺炎衣原体基因产物是沙眼衣原体迟发型超敏抗原和大肠杆菌GroEL热休克蛋白的类似物。
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