人胚肾细胞、HeLa细胞和WI38细胞对从眼部进行病毒初次分离的敏感性比较。
Comparison of the sensitivity of human embryo kidney cells, HeLa cells, and WI38 cells for the primary isolation of viruses from the eye.
作者信息
McSwiggan D A, Darougar S, Rahman A F, Gibson J A
出版信息
J Clin Pathol. 1975 May;28(5):410-3. doi: 10.1136/jcp.28.5.410.
A comparison has been made of the efficiency of human embryo kidney (HEK) cills, HeLa cells, and WI38 cells for the isolation of viruses from the eyes of patients suffering from acute conjunctivitis or keratoconjunctivitis. From a total of 99 specimens 21 adenoviruses (serotypes 3, 4, 7, 8, and 13) were isolated in HEK cells, eight (serotypes 3 and 8) in HeLa cells, and four (serotype 3) in WI38 cells. Of the ten herpes simplex viruses isolated nine were recovered in HEK cells, seven in WI38 cells, and none in HeLa cells. The combination of HeLa cells and WI38 cells is not considered an adequate alternative to the difficult-to-obtain HEK cells for the isolation of viruses from the eye.
已对人胚肾（HEK）细胞、HeLa细胞和WI38细胞从急性结膜炎或角膜结膜炎患者眼部分离病毒的效率进行了比较。在总共99份标本中，在HEK细胞中分离出21株腺病毒（血清型3、4、7、8和13），在HeLa细胞中分离出8株（血清型3和8），在WI38细胞中分离出4株（血清型3）。在分离出的10株单纯疱疹病毒中，9株在HEK细胞中分离得到，7株在WI38细胞中分离得到，在HeLa细胞中未分离到。对于从眼部分离病毒而言，HeLa细胞和WI38细胞的组合并非难以获得的HEK细胞的合适替代方案。
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