登革热病毒3型（III亚型）在印度再度出现：对登革出血热和登革休克综合征发病率上升的影响。
Reemergence of dengue virus type-3 (subtype-III) in India: implications for increased incidence of DHF & DSS.
作者信息
Dash Paban Kumar, Parida Man Mohan, Saxena Parag, Abhyankar Ajay, Singh C P, Tewari K N, Jana Asha Mukul, Sekhar K, Rao P V Lakshmana
机构信息
Division of Virology, Defence R&D Establishment, Jhansi Road, Gwalior- 474002, MP, India.
出版信息
Virol J. 2006 Jul 6;3:55. doi: 10.1186/1743-422X-3-55.
BACKGROUND
Dengue virus infection has recently taken endemic proportion in India implicating all the four known dengue serotypes. There was a major dengue outbreak in northern India including Delhi in October- December, 2003 and again in 2004. We have carried out a detailed investigation of the 2004 outbreak by Serosurveillance, RT-PCR, nested PCR, virus isolation and genotyping. We also report the molecular epidemiological investigation of these outbreaks.
RESULTS
The serological investigation of 162 suspected serum samples using an in-house dengue dipstick ELISA revealed 11%-IgM, 51%-IgG and 38%-both IgM and IgG antibody positivity. The RT-PCR analysis revealed presence of dengue RNA in 17 samples. Further subtyping and genotyping by nested PCR and nucleotide sequencing of C-prM gene junction revealed the association of subtype III of dengue virus type 3 in the outbreak.
CONCLUSION
The sudden shifting and dominance of the dengue virus serotype-3 (subtype III) replacing the earlier circulating serotype-2 (subtype IV) is a point of major concern and may be attributed to increased incidence of DHF and DSS in India.
背景
登革病毒感染最近在印度呈地方流行态势，涉及所有四种已知的登革热血清型。2003年10月至12月以及2004年，印度北部包括德里在内发生了大规模登革热疫情。我们通过血清学监测、逆转录聚合酶链反应（RT-PCR）、巢式PCR、病毒分离和基因分型对2004年的疫情进行了详细调查。我们还报告了这些疫情的分子流行病学调查情况。
结果
使用自制的登革热试纸条酶联免疫吸附测定法（ELISA）对162份疑似血清样本进行血清学调查，结果显示11%样本IgM呈阳性，51%样本IgG呈阳性，38%样本IgM和IgG抗体均呈阳性。RT-PCR分析显示17份样本中存在登革热RNA。通过巢式PCR和C-prM基因连接处的核苷酸测序进行进一步的亚型分析和基因分型，结果显示此次疫情与登革热病毒3型的III亚型有关。
结论
登革热病毒血清型3（III亚型）突然取代早期流行的血清型2（IV亚型）并占据主导地位，这是一个主要的关注点，可能是印度登革出血热（DHF）和登革休克综合征（DSS）发病率增加的原因。
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