Boudaoud L, Divaret G, Marie P, Bezeaud A
Service d'Hématologie Biologique, Hôpital Beaujon, Clichy.
Ann Biol Clin (Paris). 2006 Jul-Aug;64(4):315-7.
Routine coagulation assays are performed with platelet-poor plasma obtained after centrifugation of whole citrated blood. Usually clinical laboratories centrifuge blood from 2,000 to 2,500 g for 15-30 minutes. Thirty two blood samples routinely submitted to coagulation tests, were assayed for the prothrombin time, activated partial thromboplastin time, and fibrinogen level, in order to compare results obtained using 2 types of centrifugation : centrifugation at 2,500 g for 15 minutes and rapid centrifugation on StatSpin Express 2 at 4,440 g for 2 minutes. A good correlation was observed for the prothrombin time, activated partial thromboplastin time, and fibrinogen levels being respectively 1,009, 0,908 and 1. We concluded that rapid centrifugation at 4,440 g for 2 minutes does not modify results and contributes, by decreasing duration of the pre-analytical variable to reduce the completion time of these tests.
常规凝血检测采用全枸橼酸化血液离心后获得的乏血小板血浆进行。通常临床实验室以2000至2500克的离心力对血液离心15至30分钟。为比较两种离心方法的检测结果,对32份常规送检的凝血检测血样进行了凝血酶原时间、活化部分凝血活酶时间及纤维蛋白原水平的检测。这两种离心方法分别是:以2500克的离心力离心15分钟,以及在StatSpin Express 2离心机上以4440克的离心力快速离心2分钟。结果发现,凝血酶原时间、活化部分凝血活酶时间及纤维蛋白原水平之间具有良好的相关性,相关系数分别为1.009、0.908和1。我们得出结论,以4440克的离心力快速离心2分钟不会改变检测结果,且通过缩短分析前变量的持续时间,有助于减少这些检测的完成时间。