Pappas A A, Palmer S K, Meece D, Fink L M
Department of Pathology, University of Arkansas for Medical Sciences, Little Rock 72205.
Arch Pathol Lab Med. 1991 Aug;115(8):816-7.
Currently recommended minimum centrifugation of whole blood to produce platelet-poor plasma for routine coagulation assays is 1000g relative centrifugal force for 10 minutes. Many clinical laboratories centrifuge blood for routine coagulation assays from 500g to 2000g, with spin times varying from 20 to 5 minutes. Ninety blood samples, routinely submitted to our coagulation laboratory, were prospectively assayed simultaneously for the prothrombin time, activated partial thromboplastin time, and fibrinogen level, comparing centrifugation at 11,000g for 2 minutes with centrifugation at 1000g for 10 minutes. Routine and readily available equipment and supplies were used. Platelet counts were performed on the supernatant plasma in each sample to determine the efficacy of platelet depletion. Excellent correlation of methods was observed for the prothrombin time, activated partial thromboplastin time, and fibrinogen level. Platelet counts on the plasma supernatant showed no significant difference between the two centrifugation methods. We concluded that high-speed centrifugation at 11,000g with a shortened spin time of 2 minutes and with the use of routinely available equipment and supplies can significantly decrease the specimen preparation time for routine coagulation testing.
目前推荐的用于常规凝血检测以制备少血小板血浆的全血最低离心条件是相对离心力1000g，持续10分钟。许多临床实验室对用于常规凝血检测的血液进行离心时，离心力在500g至2000g之间，离心时间从20分钟到5分钟不等。我们前瞻性地对常规提交至我们凝血实验室的90份血样同时进行凝血酶原时间、活化部分凝血活酶时间和纤维蛋白原水平检测，比较了11,000g离心2分钟与1000g离心10分钟的效果。使用的是常规且易于获得的设备和耗材。对每个样本的上清血浆进行血小板计数，以确定血小板去除效果。观察到凝血酶原时间、活化部分凝血活酶时间和纤维蛋白原水平的检测方法具有良好的相关性。两种离心方法在血浆上清的血小板计数上无显著差异。我们得出结论，使用常规可用的设备和耗材，以11,000g高速离心且缩短至2分钟的离心时间，可显著减少常规凝血检测的标本制备时间。
