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用于常规凝血检测的血小板乏血浆的 5 分钟制备。

Five-minute preparation of platelet-poor plasma for routine coagulation testing.

机构信息

Department of Medical Laboratory Technology, College of Health Sciences, University of Sharjah, Sharjah, United Arab Emirates.

出版信息

East Mediterr Health J. 2010 Feb;16(2):233-6.

PMID:20799581
Abstract

Clinical and Laboratory Standard Institute guidelines require that routine coagulation tests are performed with platelet-poor plasma (< 10,000 platelets/microL) and prepared from whole citrated blood centrifuged at low speed for 10-30 minutes. To compare results obtained from plasma centrifuged for 5 minutes at 3000 g or for 10 minutes at 2000 g, 46 blood samples from normal healthy adults were assayed for prothrombin time, international normalized ratio and activated partial thromboplastin time. No significant differences were found in test results and it was concluded that 5 minutes centrifugation at 3000 g is a reliable and useful option to reduce the turnaround time for these tests.

摘要

临床和实验室标准协会指南要求使用血小板少于 10000 个/微升的贫血小板血浆(< 10,000 platelets/microL)并通过将全血用柠檬酸盐抗凝后以低速(10-30 分钟)离心来制备常规凝血检测。为了比较在 3000g 下离心 5 分钟或在 2000g 下离心 10 分钟获得的血浆结果,对 46 名来自正常健康成年人的血液样本进行了凝血酶原时间、国际标准化比值和活化部分凝血活酶时间检测。未发现检测结果存在显著差异,因此得出结论,3000g 下离心 5 分钟是一种可靠且有用的选择,可以缩短这些检测的周转时间。

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