Higuchi K, Kitagawa K, Naiki H, Hanada K, Hosokawa M, Takeda T
Department of Senescence Biology, Kyoto University, Japan.
Biochem J. 1991 Oct 15;279 ( Pt 2)(Pt 2):427-33. doi: 10.1042/bj2790427.
Three types of apolipoprotein A-II (apoA-II) proteins (A, B and C) were predicted from the nucleotide sequence of apoA-II cDNA. Substitution of amino acid residues was noted at four positions (type A: Pro-5, Asp-20, Met-26, Ala-38; B: Pro-5, Glu-20, Val-26, Val-38; C: Gln-5, Glu-20, Val-26, Ala-38). Each type was identifiable by digestion of amplified apoA-II DNA by PCR, using restriction-fragment-length polymorphism of the apoA-II gene for restriction enzymes Cfr13I and MspI. The molecular type of apoA-II was determined among 23 strains of mice including nine of the senescence accelerated mouse series developed in our laboratory. Examination of types of apoA-II and amyloid deposition in the F2 and F3 hybrid mice showed that apoA-II amyloid deposition was present only in the mice homozygous for type C apoA-II and which were 12-17 months of age. The molecular type of apoA-II may be a factor involved in the development of senile amyloidosis in mice.
从载脂蛋白A-II（apoA-II）cDNA的核苷酸序列预测出三种类型的载脂蛋白A-II（apoA-II）蛋白（A、B和C）。在四个位置发现了氨基酸残基的替换（A型：Pro-5、Asp-20、Met-26、Ala-38；B型：Pro-5、Glu-20、Val-26、Val-38；C型：Gln-5、Glu-20、Val-26、Ala-38）。通过使用apoA-II基因的限制性片段长度多态性，用限制性内切酶Cfr13I和MspI对通过PCR扩增的apoA-II DNA进行消化，可识别每种类型。在包括我们实验室培育的九个衰老加速小鼠品系在内的23个小鼠品系中确定了apoA-II的分子类型。对F2和F3杂交小鼠中apoA-II的类型和淀粉样蛋白沉积的检查表明，apoA-II淀粉样蛋白沉积仅存在于C型apoA-II纯合且年龄为12至17个月的小鼠中。apoA-II的分子类型可能是参与小鼠老年性淀粉样变性发展的一个因素。
