Teixeira E H, Napimoga M H, Carneiro V A, de Oliveira T M, Cunha R M S, Havt A, Martins J L, Pinto V P T, Gonçalves R B, Cavada B S
Medical School of Sobral, Federal University of Ceará, Brazil.
J Appl Microbiol. 2006 Jul;101(1):111-6. doi: 10.1111/j.1365-2672.2006.02910.x.
Initial colonization of the tooth surface by streptococci involves the attachment of these bacteria to adsorbed salivary components of the acquired pellicle. In dental biofilm this adhesion may also involve lectin-like components, present on the surface of the organisms, which bind to complementary carbohydrates on the surface of the tooth. Therefore, this work aimed to evaluate the potential of six lectins, extracted from seeds of Leguminosae family members, to inhibit the adherence of five streptococci species to acquired pellicle in vitro.
The lectins used in this work were extracted from Canavalia ensiformis, Canavalia brasiliensis, Dioclea violacea, Dioclea grandiflora, Cratylia floribunda and Vatairea macrocarpa. Fluorescence micrography was employed to visualize the ability of FITC-labeled lectins to attach to acquire pellicle. Adherence inhibition was performed on saliva-coated microtiter plates at which lectins solutions were previously incubated followed by incubation with the oral streptococci. Glucose-mannose specific lectins attached to acquired pellicle with high intensity, while galactose specific lectins, from V. macrocarpa, exhibits low intensity attachment.
All lectins were able to inhibit the adherence of the microorganisms tested (p < 0.01).
Our results suggest that lectins may be useful in anti adhesion therapeutics.
链球菌对牙面的初始定植涉及这些细菌附着于获得性膜中吸附的唾液成分。在牙生物膜中，这种黏附也可能涉及存在于生物体表面的凝集素样成分，其与牙表面的互补碳水化合物结合。因此，本研究旨在评估从豆科植物种子中提取的六种凝集素在体外抑制五种链球菌对获得性膜黏附的潜力。
本研究中使用的凝集素分别从刀豆、巴西刀豆、紫花蝶豆、大花蝶豆、多花克拉蒂亚豆和大果瓦泰豆中提取。采用荧光显微镜观察异硫氰酸荧光素标记的凝集素附着于获得性膜的能力。在预先孵育凝集素溶液的唾液包被微量滴定板上进行黏附抑制实验，随后与口腔链球菌一起孵育。葡萄糖-甘露糖特异性凝集素高强度附着于获得性膜，而来自大果瓦泰豆的半乳糖特异性凝集素附着强度较低。
所有凝集素均能抑制所测试微生物的黏附（p < 0.01）。
我们的结果表明凝集素可能在抗黏附治疗中有用。
