O'Flynn G, Coffey A, Fitzgerald G F, Ross R P
Moorepark Food Research Centre, Teagasc, Moorepark, Fermoy, Co. Cork, Ireland.
J Appl Microbiol. 2006 Jul;101(1):251-9. doi: 10.1111/j.1365-2672.2005.02792.x.
To screen Irish faecal samples from a variety of sources with a view to isolating novel anti-Salmonella phages and to subsequently evaluate their lytic capability.
Two novel anti-Salmonella phages st104a and st104b were isolated from a screening programme based on their lytic capability. The phages produced significantly larger plaques (2 mm) on the chosen indicator Salmonella enterica strain, DPC6046, when compared with the well-known control phage, Felix 01 (0.5 mm). Both phages st104a and st104b were found to have a broad host range within the Salm. enterica species. During in vitro trials, both phages (st104a and st104b) reduced Salm. enterica numbers more than 99% within 1 h. In vivo studies, involving the addition of the phage to porcine gastric juice (pH 2.5) demonstrated that phage st104a and phage Felix 01 were capable of surviving (10 and 30% survival respectively) the acidic conditions, unlike st104b, which was undetectable after 2 h exposure.
Two novel lytic anti-Salmonella phages were isolated and characterized.
With the exception of phage Felix 01, there has been relatively little phage therapy work performed using lytic Salmonella phage. In this study, the lytic phages st104a and st104b were isolated as a result of a faecal screening programme. Subsequently, phage st104a was found to have potential for biocontrol of Salm. enterica numbers if administered orally to pigs given their survival in porcine gastric juice, whereas, phage st104b may have potential in reducing cell numbers if applied by alternative approaches.
筛选来自各种来源的爱尔兰粪便样本，以期分离出新型抗沙门氏菌噬菌体，并随后评估其裂解能力。
基于其裂解能力，从一个筛选项目中分离出两种新型抗沙门氏菌噬菌体st104a和st104b。与著名的对照噬菌体Felix 01（0.5毫米）相比，这些噬菌体在选定的指示性肠炎沙门氏菌菌株DPC6046上产生的噬菌斑明显更大（2毫米）。发现噬菌体st104a和st104b在肠炎沙门氏菌物种内具有广泛的宿主范围。在体外试验中，两种噬菌体（st104a和st104b）在1小时内将肠炎沙门氏菌数量减少了99%以上。在体内研究中，将噬菌体添加到猪胃液（pH 2.5）中，结果表明噬菌体st104a和噬菌体Felix 01能够在酸性条件下存活（分别为10%和30%的存活率），而st104b在暴露2小时后无法检测到。
分离并鉴定了两种新型裂解性抗沙门氏菌噬菌体。
除了噬菌体Felix 01外，使用裂解性沙门氏菌噬菌体进行的噬菌体治疗工作相对较少。在本研究中，通过粪便筛选项目分离出了裂解性噬菌体st104a和st104b。随后发现，如果口服给猪，噬菌体st104a因其能在猪胃液中存活而具有控制肠炎沙门氏菌数量的潜力，而噬菌体st104b如果通过其他途径应用，可能具有减少细胞数量的潜力。
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