[喉鳞状细胞癌中尿激酶型纤溶酶原激活剂启动子的甲基化状态]

[Methylation status of uPA promoter in laryngeal squamous cell carcinoma].

作者信息

Wang Dali, Wang Tie, Ren Zhong

机构信息

Department of Otorhinolaryngology, the Second Affiliated Hospital, China Medical University, Shenyang.

出版信息

Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2006 May;20(9):409-11.

PMID:16836076

Abstract

OBJECTIVE

To investigate the relationship between methylation status of urokinase type plasminogen activator (uPA) promoter and invasion/metastasis of laryngeal squamous cell carcinoma (LSCC).

METHOD

Using RT-PCR technique, specimens from 40 patients of LSCC were detected for their expression of the uPA gene. Meanwhile, methylation-specific PCR was used to detect methylation status of uPA promoter.

RESULT

Positive expression rate of uPAmRNA in LSCC was 65.0% (26/40), and the rate of expression of uPAmRNA in LSCC with cervical lymph node metastasis was higher than that of in LSCC without cervical lymph node metastasis (P <0.05). Methylation of uPA promoter was observed in 8 (20.0%) of 40 cases of LSCC, and the rate of methylation of uPA promoter in LSCC with cervical lymph node metastasis was lower than that of in LSCC without cervical lymph node metastasis (P <0.01). The expression of uPAmRNA was not detected in methylated cancer tissues.

CONCLUSION

Methylation leads to lose of the uPA gene expression in LSCC. Demethylation of uPA promoter may be correlated to cervical lymph node metastasis.

摘要

目的

探讨尿激酶型纤溶酶原激活剂（uPA）启动子甲基化状态与喉鳞状细胞癌（LSCC）侵袭/转移的关系。

方法

采用逆转录-聚合酶链反应（RT-PCR）技术检测40例LSCC患者标本中uPA基因的表达。同时，用甲基化特异性PCR检测uPA启动子的甲基化状态。

结果

LSCC中uPA mRNA阳性表达率为65.0%（26/40），有颈部淋巴结转移的LSCC中uPA mRNA表达率高于无颈部淋巴结转移的LSCC（P<0.05）。40例LSCC中有8例（20.0%）观察到uPA启动子甲基化，有颈部淋巴结转移的LSCC中uPA启动子甲基化率低于无颈部淋巴结转移的LSCC（P<0.01）。甲基化的癌组织中未检测到uPA mRNA表达。