Matchett Gerald, Hahn Joseph, Obenaus Andre, Zhang John
Department of Anesthesiology, Loma Linda University, School of Medicine, Loma Linda, CA, United States.
J Neurosci Methods. 2006 Dec 15;158(2):234-41. doi: 10.1016/j.jneumeth.2006.06.003. Epub 2006 Jul 11.
Brain tissue at the edge of a surgical resection site is at risk for damage from direct trauma, retractor stretch, hemorrhage, edema, and electrocautery. In this study we used a new rodent model of surgically induced brain injury (SBI) to study this tissue at the edge of a resection site. The SBI model entails stereotaxic resection of part of the right frontal lobe. We tested pretreatment with erythropoietin, a known neuroprotectant, for protective effects in this model. Three groups of male Sprague-Dawley rats (280-330g) were used: SBI without treatment (n=63), SBI with EPO treatment (n=76), and Sham surgery (n=12). Rats were sacrificed 24h, 72h, and 7 days after SBI or Sham surgery. Postoperative assessment included mortality, histology, immunohistochemistry, Evans blue exudation, brain water content, and magnetic resonance imaging. No difference was found between untreated and EPO-treated groups in mortality, histology, TUNEL, magnetic resonance imaging, or blood-brain-barrier breakdown. The EPO-treated group had statistically more brain water content at 24h than the untreated group. Immunohistochemistry demonstrated a qualitative increase in VEGF in the EPO-treatment group. We conclude that EPO does not ameliorate damage in SBI, and may increase brain edema early after surgery.
手术切除部位边缘的脑组织有遭受直接创伤、牵开器拉伸、出血、水肿和电灼损伤的风险。在本研究中，我们使用一种新的手术诱导脑损伤（SBI）啮齿动物模型来研究切除部位边缘的这种组织。SBI模型需要对右侧额叶的一部分进行立体定向切除。我们测试了用已知的神经保护剂促红细胞生成素进行预处理在该模型中的保护作用。使用了三组雄性Sprague-Dawley大鼠（280-330g）：未治疗的SBI组（n=63）、促红细胞生成素治疗的SBI组（n=76）和假手术组（n=12）。在SBI或假手术后24小时、72小时和7天处死大鼠。术后评估包括死亡率、组织学、免疫组织化学、伊文思蓝渗出、脑含水量和磁共振成像。在死亡率、组织学、TUNEL、磁共振成像或血脑屏障破坏方面，未治疗组和促红细胞生成素治疗组之间未发现差异。促红细胞生成素治疗组在24小时时的脑含水量在统计学上高于未治疗组。免疫组织化学显示促红细胞生成素治疗组中血管内皮生长因子有定性增加。我们得出结论，促红细胞生成素不能改善SBI中的损伤，并且可能在手术后早期增加脑水肿。
