Nery Susana, Deans Anne-Marie, Mosobo Moses, Marsh Kevin, Rowe J Alexandra, Conway David J
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom.
Mol Biochem Parasitol. 2006 Oct;149(2):208-15. doi: 10.1016/j.molbiopara.2006.05.014. Epub 2006 Jun 22.
Plasmodium falciparum merozoites invade erythrocytes using a range of alternative ligands that includes erythrocyte binding antigenic proteins (EBAs) and reticulocyte binding protein homologues (Rh). Variation in the expression of some of these genes among culture-adapted parasite lines correlates with the use of different erythrocyte receptors. Here, expression profiles of four Rh genes and eba175 are analysed in a sample of 42 isolates cultured from malaria patients in Kenya. The profiles cluster into distinct groups, largely because of very strong negative correlations between the levels of expression of particular gene pairs (Rh1 versus Rh2b, eba175 versus Rh2b, and eba175 versus Rh4), previously associated with alternative invasion pathways in culture-adapted parasite lines. High levels of eba175 are seen in isolates in expression profile group I, and may be associated with sialic acid-dependent invasion. Groups II and III are, respectively, characterized by high levels of Rh2b and Rh4, and are more likely to be associated with sialic acid-independent invasion.
恶性疟原虫裂殖子利用一系列替代配体侵入红细胞，这些配体包括红细胞结合抗原蛋白（EBAs）和网织红细胞结合蛋白同源物（Rh）。在适应培养的寄生虫株系中，其中一些基因表达的变化与不同红细胞受体的使用相关。在此，对从肯尼亚疟疾患者中培养的42个分离株样本中的4个Rh基因和eba175的表达谱进行了分析。这些表达谱聚为不同的组，主要是因为特定基因对（Rh1与Rh2b、eba175与Rh2b以及eba175与Rh4）表达水平之间存在非常强的负相关，这在适应培养的寄生虫株系中先前与替代侵入途径相关。在表达谱I组的分离株中观察到高水平的eba175，并且可能与唾液酸依赖性侵入相关。II组和III组分别以高水平的Rh2b和Rh4为特征，并且更可能与唾液酸非依赖性侵入相关。
