Department of Applied Genomics, Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, H-2462 Martonvásár, Brunszvik, Hungary.
PLoS One. 2012;7(12):e52688. doi: 10.1371/journal.pone.0052688. Epub 2012 Dec 20.
Potential porcine circovirus type 2 (PCV2) capsid protein epitopes, suitable for expression on the surface of cucumber mosaic virus (CMV) particles were determined by a thorough analysis of the predicted PCV capsid protein structure. The ab initio protein structure prediction was carried out with fold recognition and threading methods. The putative PCV epitopes were selected on the basis of PCV virion models and integrated into the plant virus coat protein, after amino acid position 131. The recombinants were tested for infectivity and stability on different Nicotiana species and stable recombinant virus particles were purified. The particles were tested for their ability to bind to PCV induced porcine antibodies and used for specific antibody induction in mice and pigs. The results showed that PCV epitopes expressed on the CMV surface were recognized by the porcine antibodies and they were also able to induce PCV specific antibody response. Challenge experiment with PCV2 carried out in immunized pigs showed partial protection against the infection. Based on these results it was concluded that specific antiviral vaccine production for the given pathogen was feasible, offering an inexpensive way for the mass production of such vaccines.
通过对预测的猪圆环病毒 2 型（PCV2）衣壳蛋白结构的深入分析，确定了适合在黄瓜花叶病毒（CMV）粒子表面表达的潜在 PCV2 衣壳蛋白表位。使用折叠识别和穿线方法进行了从头蛋白结构预测。基于 PCV 病毒粒子模型选择了推定的 PCV 表位，并将其整合到植物病毒外壳蛋白中，位于第 131 位氨基酸之后。在不同的烟草属物种上测试了重组体的感染性和稳定性，并纯化了稳定的重组病毒粒子。测试了这些粒子与 PCV 诱导的猪抗体结合的能力，并在小鼠和猪中用于诱导特异性抗体。结果表明，在 CMV 表面表达的 PCV 表位被猪抗体识别，并且还能够诱导 PCV 特异性抗体反应。在免疫猪中进行的 PCV2 攻毒实验表明，该方法对感染具有部分保护作用。基于这些结果，可以得出结论，针对特定病原体生产特异性抗病毒疫苗是可行的，为大规模生产此类疫苗提供了一种廉价的方法。
