Huang E S
J Virol. 1975 Aug;16(2):298-310. doi: 10.1128/JVI.16.2.298-310.1975.
Infection of WI-38 human fibroblasts with human cytomegalovirus (CMV) led to the stimulation of host cell DNA polymerase synthesis and induction of a novel virus-specific DNA polymerase. This cytomegalovirus-induced DNA polymerase was purified and separated from host cell enzymes by DEAE-cellulose and phosphocellulose column chromatographies. It can be distinguished from host cell enzymes by chromatographic behavior, template primer specificity, sedimentation property, and the requirement of salt for maximal activity. This virus-induced enzyme has a sedimentation coefficient of 9.2S and is found in both the nuclei and cytoplasm of virus-infected cells, but not in uninfected cells. This enzyme could efficiently use activated calf-thymus DNA, oly(dA)-oligo(dT)12-18, and poly(dC)-oligo(dG)12-18 as template primers, especially poly(dA)-oligo(dT)12-18, but it could not use poly(rA)-oligo(dT)12-18, poly(rC)-oligo(dG)12-18, or oligo(dT)12-18. The enzyme requires Mg2+ for maximal activity, is sensitive to p-hydroxymercuribenzoate, and is not a zinc metalloenzyme. In addition, the cytomegalovirus-induced DNA polymerase activity can be enhanced by adding 0.06 to 0.12 M NaCl or 0.03 to 0.06 M (NH4)2SO4 to the reaction mixture.
用人巨细胞病毒（CMV）感染WI - 38人成纤维细胞，可刺激宿主细胞DNA聚合酶的合成，并诱导一种新型病毒特异性DNA聚合酶的产生。这种巨细胞病毒诱导的DNA聚合酶通过DEAE - 纤维素和磷酸纤维素柱色谱法进行纯化，并与宿主细胞酶分离。它可通过色谱行为、模板引物特异性、沉降特性以及最大活性对盐的需求与宿主细胞酶区分开来。这种病毒诱导的酶沉降系数为9.2S，存在于病毒感染细胞的细胞核和细胞质中，但在未感染细胞中不存在。该酶能有效利用活化的小牛胸腺DNA、聚（dA）- 寡聚（dT）12 - 18和聚（dC）- 寡聚（dG）12 - 18作为模板引物，尤其是聚（dA）- 寡聚（dT）12 - 18，但不能利用聚（rA）- 寡聚（dT）12 - 18、聚（rC）- 寡聚（dG）12 - 18或寡聚（dT）12 - 18。该酶最大活性需要Mg2 +，对对羟基汞苯甲酸敏感，且不是锌金属酶。此外，向反应混合物中添加0.06至0.12 M NaCl或0.03至0.06 M（NH4）2SO4可增强巨细胞病毒诱导的DNA聚合酶活性。
