鸡胚DNA聚合酶的引物需求和模板特异性
Primer requirement and template specificity of a DNA polymerase of chick embryo.
作者信息
Rougeon F, Brun G, da Costa Maia J C, Chapeville F
出版信息
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1229-33. doi: 10.1073/pnas.70.4.1229.
A DNA polymerase purified from extracts of chick embryo catalyzes the synthesis of poly(dT) in the presence of poly(rA) or poly(dA) as template and poly(dT) or oligo(dT) as primer. Oligo- or polyribonucleotides are relatively ineffective initiators of polydeoxynucleotide polymerization. Using activated DNA as template, the enzyme catalyzes a repair-like reaction that resembles that catalyzed by RNA-directed DNA polymerase of RNA tumor viruses and Escherichia coli DNA polymerase II. With natural templates containing poly(rA) sequences, such as avian myeloblastosis virus RNA or rabbit globin mRNA, and oligo(dT) as initiator, principally poly(dT) is synthesized. Consequently, the enzyme differs from the RNA-directed DNA polymerase associated with RNA tumor viruses by its incapacity to transcribe heteropolymeric regions of RNA templates. The possible role of the enzyme in repair of DNA during transcription is discussed.
从鸡胚提取物中纯化得到的一种DNA聚合酶，在以聚（rA）或聚（dA）为模板、聚（dT）或寡聚（dT）为引物的情况下，催化聚（dT）的合成。寡聚或多聚核糖核苷酸作为多脱氧核苷酸聚合的起始物相对无效。以活化的DNA为模板时，该酶催化一种类似修复的反应，类似于RNA肿瘤病毒的RNA指导的DNA聚合酶和大肠杆菌DNA聚合酶II所催化的反应。以含有聚（rA）序列的天然模板，如禽成髓细胞瘤病毒RNA或兔珠蛋白mRNA，以及寡聚（dT）为起始物时，主要合成聚（dT）。因此，该酶与RNA肿瘤病毒相关的RNA指导的DNA聚合酶不同，它无法转录RNA模板的异聚区域。文中讨论了该酶在转录过程中DNA修复中的可能作用。
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