Mar E C, Huang Y S, Huang E S
J Virol. 1978 May;26(2):249-56. doi: 10.1128/JVI.26.2.249-256.1978.
Infection of WI-38 human fibroblasts with varicella-zoster virus led to the stimulation of host cell DNA polymerase synthesis and induction of a new virus-specific DNA polymerase. This virus-induced DNA polymerase was partially purified and separated from host cell enzymes by DEAE-cellulose and phosphocellulose column chromatographies. This virus-induced enzyme could be distinguished from host cell enzyme by its chromatographic behavior, template specificity, and its requirement of salt for maximal activity. The enzyme could efficiently use poly(dC).oligo(dG)12-18 as well as poly(dA).oligo(dT)12-18 as template-primers. It required Mg2+ for maximal polymerization activity and was sensitive to phosphonoacetic acid, to which host alpha- and beta-DNA polymerase were relatively resistant. In addition, this induced DNA polymerase activity was enhanced by adding 60 mM (NH4)2SO4 to the reaction mixture.
水痘-带状疱疹病毒感染WI-38人成纤维细胞会刺激宿主细胞DNA聚合酶的合成，并诱导一种新的病毒特异性DNA聚合酶产生。这种病毒诱导的DNA聚合酶经过部分纯化，并通过DEAE-纤维素和磷酸纤维素柱色谱法与宿主细胞酶分离。这种病毒诱导的酶可通过其色谱行为、模板特异性以及其最大活性对盐的需求与宿主细胞酶区分开来。该酶能高效地将聚（dC）·寡聚（dG）12 - 18以及聚（dA）·寡聚（dT）12 - 18用作模板引物。它需要Mg2+来实现最大聚合活性，并且对膦乙酸敏感，而宿主α-和β-DNA聚合酶对膦乙酸相对耐药。此外，向反应混合物中添加60 mM硫酸铵可增强这种诱导的DNA聚合酶活性。
