鸡胚DNA聚合酶：纯化及性质
DNA polymerase of chicken embryo: purification and properties.
作者信息
Stavrianopoulos J G, Karkas J D, Chargaff E
出版信息
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1781-5. doi: 10.1073/pnas.69.7.1781.
Abstract
The almost complete purification of the previously reported DNA polymerase of chicken embryo is described; in the final isolation step the enrichment of the enzyme, which comprises about 0.01% of the protein extractable from 10-day-old embryos, is more than 9000-fold. The enzyme is a basic protein (pI = 9.15), of molecular weight about 27,000. It exhibits no nuclease activity, requires Mn(2+) ion for activity, and may contain Zn. It is very sensitive to oxidation.
摘要
本文描述了鸡胚中先前报道的DNA聚合酶几乎完全纯化的过程；在最终的分离步骤中，该酶（占10日龄胚胎可提取蛋白质的约0.01%）的富集倍数超过9000倍。该酶是一种碱性蛋白（pI = 9.15），分子量约为27000。它不具有核酸酶活性，活性需要Mn(2+)离子，可能还含有锌。它对氧化非常敏感。
相似文献
1
DNA polymerase of chicken embryo: purification and properties.鸡胚DNA聚合酶：纯化及性质
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1781-5. doi: 10.1073/pnas.69.7.1781.
2
Purification and properties of DNA polymerases from chick embryo.鸡胚DNA聚合酶的纯化及性质
Eur J Biochem. 1974 Jan 16;41(2):241-51. doi: 10.1111/j.1432-1033.1974.tb03265.x.
3
Purification of a DNA polymerase from calf thymus nuclei.从小牛胸腺细胞核中纯化一种DNA聚合酶。
Biochim Biophys Acta. 1972 Jul 31;272(4):526-38. doi: 10.1016/0005-2787(72)90508-4.
4
[Polyadenylate synthetase from mouse ascites cells. I. Purification and characterization of the enzyme].[来自小鼠腹水细胞的聚腺苷酸合成酶。I. 酶的纯化与特性分析]
Biochim Biophys Acta. 1972 Dec 22;287(3):438-47.
5
Fructose 1,6-diphosphatase from Rhodopseudomonas palustris. I. Purification and properties.来自沼泽红假单胞菌的果糖1,6 -二磷酸酶。I. 纯化及性质
Arch Biochem Biophys. 1972 Sep;152(1):1-12. doi: 10.1016/0003-9861(72)90186-5.
6
Studies on trypsin inhibitors in sweet potato. I. Purification and some properties.甘薯中胰蛋白酶抑制剂的研究。I. 纯化及某些性质
Biochim Biophys Acta. 1973 Dec 6;328(2):407-17. doi: 10.1016/0005-2795(73)90275-4.
7
Partial purification of a chloroplast DNA polymerase from Euglena gracilis.纤细裸藻叶绿体DNA聚合酶的部分纯化
Biochem Biophys Res Commun. 1973 Feb 5;50(3):620-8. doi: 10.1016/0006-291x(73)91289-8.
8
Purification and properties of deoxyribonucleic acid polymerase from nuclei of sea urchin embryos.海胆胚胎细胞核中脱氧核糖核酸聚合酶的纯化及性质
J Biol Chem. 1969 Apr 10;244(7):1672-81.
9
Purification and some properties of a factor (S) blocking the synthesis of covalently linked complementary sequences by deoxyribonucleic acid polymerase I.一种抑制脱氧核糖核酸聚合酶I合成共价连接互补序列的因子（S）的纯化及其某些性质
Biochemistry. 1974 Apr 9;13(8):1610-4. doi: 10.1021/bi00705a009.
10
Solubilization and characterization of particulate form of DNA polymerase from adult rat liver nuclei.成年大鼠肝细胞核中DNA聚合酶颗粒形式的溶解与特性分析
Biochem Biophys Res Commun. 1972 Mar 10;46(5):1830-6. doi: 10.1016/0006-291x(72)90058-7.
引用本文的文献
1
Hepatitis-B virus-associated deoxyribonucleic acid polymerase: a partial characterization by the use of chemical agents.乙型肝炎病毒相关脱氧核糖核酸聚合酶：利用化学试剂进行的部分特性鉴定
Klin Wochenschr. 1981 Jun 15;59(12):691-7. doi: 10.1007/BF02593862.
2
Purification and properties of ribonuclease H of calf thymus.小牛胸腺核糖核酸酶H的纯化及性质
Proc Natl Acad Sci U S A. 1973 Jul;70(7):1959-63. doi: 10.1073/pnas.70.7.1959.
3
Mechanism of DNA replication by highly purified DNA polymerase of chicken embryo.鸡胚高度纯化的DNA聚合酶进行DNA复制的机制
Proc Natl Acad Sci U S A. 1972 Sep;69(9):2609-13. doi: 10.1073/pnas.69.9.2609.
4
Survey of DNA polymerase activity during the early development of Drosophila melanogaster.黑腹果蝇早期发育过程中DNA聚合酶活性的研究
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2946-50. doi: 10.1073/pnas.70.10.2946.
5
RNA-directed DNA-polymerase activity in Greene hamster melanoma.格林仓鼠黑色素瘤中的RNA指导的DNA聚合酶活性
Yale J Biol Med. 1973 Dec;46(5):485-91.
6
Reverse transcription by Escherichia coli DNA polymerase I.大肠杆菌DNA聚合酶I介导的逆转录
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3834-8. doi: 10.1073/pnas.70.12.3834.
7
Primer requirement and template specificity of a DNA polymerase of chick embryo.鸡胚DNA聚合酶的引物需求和模板特异性
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1229-33. doi: 10.1073/pnas.70.4.1229.
8
Inhibition of RNA-dependent DNA polymerase of Rous sarcoma virus by thiosemicarbazones and several cations.硫代氨基脲和几种阳离子对劳氏肉瘤病毒RNA依赖性DNA聚合酶的抑制作用。
Proc Natl Acad Sci U S A. 1973 Jan;70(1):164-8. doi: 10.1073/pnas.70.1.164.
9
A low molecular weight DNA polymerase from wheat embryos.一种来自小麦胚的低分子量DNA聚合酶。
Plant Mol Biol. 1990 Sep;15(3):383-97. doi: 10.1007/BF00019156.
10
Ribonuclease H of calf thymus: substrate specificity, activation, inhibition.小牛胸腺核糖核酸酶H：底物特异性、激活与抑制
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1087-91. doi: 10.1073/pnas.73.4.1087.
本文引用的文献
1
Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.碱性蛋白质和肽在聚丙烯酰胺凝胶上的圆盘电泳。
Nature. 1962 Jul 21;195:281-3. doi: 10.1038/195281a0.
2
A method for determining the sedimentation behavior of enzymes: application to protein mixtures.一种测定酶沉降行为的方法：应用于蛋白质混合物
J Biol Chem. 1961 May;236:1372-9.
3
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
4
Doxynucleotide-polymerizing enzymes of calf thymus gland. IV. Inhibition of terminal deoxynucleotidyl transferase by metal ligands.小牛胸腺的脱氧核苷酸聚合酶。IV. 金属配体对末端脱氧核苷酸转移酶的抑制作用
Proc Natl Acad Sci U S A. 1970 Apr;65(4):1041-8. doi: 10.1073/pnas.65.4.1041.
5
Nucleic acid polymerases of the developing chicken embryo: a DNA polymerase preferring a hybrid template.发育中的鸡胚核酸聚合酶：一种偏好杂交模板的DNA聚合酶。
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2207-11. doi: 10.1073/pnas.68.9.2207.
6
Zinc in DNA polymerases.DNA聚合酶中的锌
Biochem Biophys Res Commun. 1971 Jul 2;44(1):37-43. doi: 10.1016/s0006-291x(71)80155-9.
7
Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase.脱氧核糖核酸的酶促合成。二十六。一种纯一的脱氧核糖核酸聚合酶的物理和化学研究。
J Biol Chem. 1969 Jun 10;244(11):2996-3008.
8
Estimation of the molecular weights of proteins by Sephadex gel-filtration.用葡聚糖凝胶过滤法估算蛋白质的分子量
Biochem J. 1964 May;91(2):222-33. doi: 10.1042/bj0910222.
Zotero 插件