McGregor J M, Barker J N, Allen M H, MacDonald D M
Laboratory of Applied Dermatopathology, United Medical School, (Guy's Campus), London, U.K.
Br J Dermatol. 1991 Nov;125(5):413-8. doi: 10.1111/j.1365-2133.1991.tb14765.x.
The demonstration of HLA-DR on human acrosyringium has led to the suggestion that eccrine epithelium, through its interaction with certain molecules, might play an active role in epidermal immune responses. An immunohistochemical study was undertaken to identify the antigenic profile of acrosyringium in normal skin and following the intradermal administration of a T-lymphocyte-derived cytokine, interferon gamma (IFN-gamma). Acrosyringium in normal skin, in contrast to interappendageal epidermis, was found to lack CD1a+ Langerhans cells. However, antigens CD36 (OKM5) and L1 (MAC387) were uniquely expressed by keratinocytes immediately adjacent to the distal portion of acrosyringium. Constitutive expression of each class II MHC antigen, namely HLA-DR, DP and DQ was observed on luminal acrosyringial cells. EMB11 antigen (CD68), a mononuclear cell determinant, was similarly expressed on acrosyringial epithelium in normal skin. Following intradermal administration of IFN-gamma, intercellular adhesion molecule-1 (ICAM-1) (CD54) was induced on acrosyringial epithelium and the expression of HLA-DR was intensified. A range of other markers including CD3, CD4, CD8, CD11a, CD11b and CD15 were not expressed by acrosyringium either in normal skin or after administration of IFN-gamma. Expression of antigens associated with cell-mediated immune mechanisms on acrosyringium is consistent with the hypothesis that it may have an immunological role in epidermal immune responses.
人类顶泌汗腺导管上HLA - DR的证实提示，小汗腺上皮细胞通过与某些分子相互作用，可能在表皮免疫反应中发挥积极作用。开展了一项免疫组织化学研究，以确定正常皮肤以及皮内注射T淋巴细胞衍生的细胞因子γ干扰素（IFN - γ）后顶泌汗腺导管的抗原谱。与附属器间表皮不同，正常皮肤中的顶泌汗腺导管未发现CD1a + 朗格汉斯细胞。然而，紧邻顶泌汗腺导管远端的角质形成细胞特异性表达抗原CD36（OKM5）和L1（MAC387）。在顶泌汗腺导管管腔细胞上观察到了II类主要组织相容性复合体（MHC）各抗原，即HLA - DR、DP和DQ的组成性表达。单核细胞决定簇EMB11抗原（CD68）在正常皮肤的顶泌汗腺导管上皮也有类似表达。皮内注射IFN - γ后，顶泌汗腺导管上皮诱导表达细胞间黏附分子 - 1（ICAM - 1）（CD54），且HLA - DR的表达增强。包括CD3、CD4、CD8、CD11a、CD11b和CD15在内的一系列其他标志物在正常皮肤或注射IFN - γ后的顶泌汗腺导管中均未表达。顶泌汗腺导管上与细胞介导免疫机制相关抗原的表达与它可能在表皮免疫反应中具有免疫作用这一假说一致。
