Murphy G F, Shepard R S, Harrist T J, Bronstein B R, Bhan A K
J Invest Dermatol. 1983 Aug;81(2):181-3. doi: 10.1111/1523-1747.ep12543766.
We have observed that monoclonal antibodies directed against human Ia-like antigens react with a subset of epidermal keratinocytes as well as Langerhans cells in normal human skin. Membrane reactivity for HLA-DR antigen in flattened ductal keratinocytes and in adjacent cuticular cells forming the acrosyringial lumen was observed using immunoelectron microscopy. It should be recognized that Ia-positive acrosyringial keratinocytes represent a potential source of contamination in methods designed to study and isolate Langerhans cells using antibodies directed against HLA-DR antigens. Teleologic considerations of HLA-DR antigen expression in the acrosyringium are discussed.
我们观察到，针对人类Ia样抗原的单克隆抗体可与正常人皮肤中的一部分表皮角质形成细胞以及朗格汉斯细胞发生反应。使用免疫电子显微镜观察到扁平导管角质形成细胞和形成顶泌汗腺管腔的相邻角质层细胞中HLA-DR抗原的膜反应性。应该认识到，Ia阳性的顶泌汗腺角质形成细胞是在使用针对HLA-DR抗原的抗体来研究和分离朗格汉斯细胞的方法中潜在的污染来源。本文讨论了顶泌汗腺中HLA-DR抗原表达的目的论考量。
