Okada M, Yamashita Y, Oseto M, Shinozaki K
Division of Virology, Chiba Prefectural Institute of Public Health, Chuo-ku, Chiba-shi, Chiba, Japan.
Arch Virol. 2006 Dec;151(12):2503-9. doi: 10.1007/s00705-006-0820-1. Epub 2006 Jul 19.
Sapovirus (SV) causes gastroenteritis in humans and comprises genetically divergent viruses. A nested reverse transcription-polymerase chain reaction (RT-PCR) targeting the capsid-protein-coding region was developed using universal and genogroup-specific primer sets. The universal primers were capable of detecting human SV genogroups I, II, IV and V. Genetic analysis of the amplified products enabled us to phylogenetically determine the genotypes of the viruses. In addition, genogroup-specific primers that amplified different lengths of the amplicon depending on the genogroup were developed. These genogroup-specific primers were also used as inner primers for the nested PCR. These two simple RT-PCR methods are powerful tools for both detection and epidemiological studies of human SV.
札幌病毒（SV）可引起人类肠胃炎，且包含基因差异较大的病毒。利用通用引物组和基因群特异性引物组，开发了一种针对衣壳蛋白编码区的巢式逆转录聚合酶链反应（RT-PCR）。通用引物能够检测人类SV基因群I、II、IV和V。对扩增产物进行基因分析，使我们能够从系统发育角度确定病毒的基因型。此外，还开发了基因群特异性引物，这些引物根据基因群扩增出不同长度的扩增子。这些基因群特异性引物也用作巢式PCR的内引物。这两种简单的RT-PCR方法是人类SV检测和流行病学研究的有力工具。
