Akihara Shiho, Phan Tung Gia, Nguyen Tuan Anh, Yagyu Fumihiro, Okitsu Shoko, Müller Werner E G, Ushijima Hiroshi
Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
J Med Virol. 2005 Dec;77(4):595-601. doi: 10.1002/jmv.20497.
A total of 921 fecal specimens collected from 44 infants in a day care center in Tokyo, Japan during June 1999 to July 2000 were tested for the presence of sapovirus by reverse transcription-polymerase chain reaction (RT-PCR). Of 88 fecal specimens from infants with acute gastroenteritis, 2.3% (2) were found to be positive for sapovirus. Twenty-two of 833 (2.6%) fecal specimens collected from asymptomatic infants were also infected with this virus. Another interesting feature was the demonstration of high incidence of sapovirus infection (95.5%, 21 of 22) identified in a single day care center, which was not due to viral shedding after the latest acute gastroenteritis. Sapovirus was subjected to genetic analysis by sequencing. Up to 4 of 24 sapoviruses (16.7%) were clustered into genogroup IV known as a rare group. Remarkably, majority (79.2%, 19 of 24) of sapovirus isolates detected in this study turned out to present one novel sapovirus genotype tentatively called GI/8. It was noteworthy to point out that the GI/8 sapovirus infection was apparently confined only within the period of 9 weeks (44th through 52nd weeks). This pattern of infection implied the outbreak of asymptomatic GI/8 sapovirus infection in these subjects. The findings clearly indicate genogroup I sapovirus can be classified into eight genotypes. This is the first report to underscore that sapovirus pathogen causes not only clinical manifestations of acute gastroenteritis but also asymptomatic infection in infants in day care centers in Japan.
1999年6月至2000年7月期间,从日本东京一家日托中心的44名婴儿身上采集了共计921份粪便标本,采用逆转录聚合酶链反应(RT-PCR)检测是否存在萨波病毒。在88份患有急性肠胃炎婴儿的粪便标本中,2.3%(2份)被检测出萨波病毒呈阳性。从无症状婴儿身上采集的833份粪便标本中,有22份(2.6%)也感染了这种病毒。另一个有趣的特征是,在一个日托中心发现萨波病毒感染的发生率很高(95.5%,22份中的21份),这并非是最近一次急性肠胃炎后的病毒排泄所致。对萨波病毒进行了测序的基因分析。24份萨波病毒中,多达4份(1