Pietruchinski Eduardo, Benati Fabrício, Lauretti Flávio, Kisielius Jonas, Ueda Marli, Volotão Eduardo M, Soares Caroline C, Hoshino Yasutaka, Linhares Rosa Elisa C, Nozawa Carlos, Santos Norma
Departamento de Microbiologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, PR, Brazil.
J Med Virol. 2006 Sep;78(9):1241-9. doi: 10.1002/jmv.20686.
Between May and August in 2003, a total of 251 fecal samples were collected from children and adults with diarrhea (5 inpatients and 246 outpatients) at a private hospital in the city of Ponta Grossa, the state of Paraná, Brazil. Group A rotavirus was detected in 71 of 251 (28.3%) specimens: 55 (77.5%) from children under 5 years of age and 16 (22.5%) from individuals aged 6-72 years. All 71 strains exhibited a "long" RNA pattern when analyzed by PAGE. Sixty-one positive samples that yielded enough RNA were submitted to PCR genotyping. The most frequent G/P genotype combination detected was G1P[8] (86.9%; 53/61) followed by G9P[8] (3.3%; 2/61) and G12P[9] (1.6%; 1/61). Rotaviruses with G2, G3, G4, P[4], or P[6] specificity were not detected. For three strains (4.9%) bearing G1 genotype, the VP4 specificity could no be determined, and two specimens (3.3%) remained G/P non-typeable. One rotavirus strain (HC91) bearing G12P[9] genotype with a "long" electropherotype was isolated from an 11-month-old boy with diarrhea for the first time in Brazil. The cell-culture grown HC91 strain was shown to belong to serotype G12 by neutralization.
2003年5月至8月期间，在巴西巴拉那州蓬塔格罗萨市的一家私立医院，共采集了251份腹泻儿童和成人（5名住院患者和246名门诊患者）的粪便样本。251份样本中有71份（28.3%）检测出A组轮状病毒：55份（77.5%）来自5岁以下儿童，16份（22.5%）来自6至72岁个体。通过聚丙烯酰胺凝胶电泳（PAGE）分析，所有71株病毒均呈现“长”RNA图谱。61份产生足够RNA的阳性样本进行了PCR基因分型。检测到的最常见G/P基因型组合是G1P[8]（86.9%；53/61），其次是G9P[8]（3.3%；2/61）和G12P[9]（1.6%；1/61）。未检测到具有G2、G3、G4、P[4]或P[6]特异性的轮状病毒。对于3株（4.9%）携带G1基因型的病毒，VP4特异性无法确定，2份样本（3.3%）仍无法进行G/P分型。从一名11个月大腹泻男童中首次分离出一株带有G12P[9]基因型且具有“长”电泳图谱的轮状病毒株（HC91）。通过中和试验表明，细胞培养的HC91株属于G12血清型。
