Li Pei, Ling Zhiqiang, Yang Hongyan, Huang Youtian, Zhao Mingyao, Zheng Zhimin, Dong Ziming
Department of Pathophysiology, College of Medical Sciences, Zhengzhou University, China.
J Huazhong Univ Sci Technolog Med Sci. 2006;26(2):167-71. doi: 10.1007/BF02895806.
The differentially expressed genes between esophageal squamous cell carcinoma (ESCC) with or without lymphatic metastasis were investigated by gene chip, and the lymphatic metastasis-associated genes were screened out. Expression array was used to detect the mRNA from both the primary carcinoma and the corresponding esophageal epithelium in 15 cases of human ESCC. The lymphatic metastasis-associated genes were screened by bioinformatics between ESCC with or without lymphatic metastasis. The results showed that 43 (4.85 %) genes significantly differed between the ESCC with and without lymphatic metastasis (P<0.05), of which 18 (2.03 %)were upregulated and 25 (2.82 %) down-regulated. The up-regulated genes were involved in cell adhesion molecules and cell membrane receptors and the down-regulated genes were mostly cell cycle regulators and intracellular signaling molecules. It was suggested that lymphatic metastasis-associated genes were screened by gene chip, which was helpful to understand the molecular mechanism of ESCC lymphatic metastasis and lymphatic metastasis-associated genes might be used as diagnostic markers and therapeutic targets for lymphatic metastasis.
通过基因芯片研究有或无淋巴结转移的食管鳞状细胞癌（ESCC）之间的差异表达基因，并筛选出淋巴结转移相关基因。采用表达谱芯片检测15例人ESCC原发癌组织及相应食管上皮组织的mRNA。通过生物信息学方法筛选有或无淋巴结转移的ESCC之间的淋巴结转移相关基因。结果显示，有和无淋巴结转移的ESCC之间有43个（4.85%）基因存在显著差异（P<0.05），其中18个（2.03%）上调，25个（2.82%）下调。上调基因涉及细胞黏附分子和细胞膜受体，下调基因大多为细胞周期调节因子和细胞内信号分子。提示通过基因芯片筛选出淋巴结转移相关基因，有助于了解ESCC淋巴结转移的分子机制，且淋巴结转移相关基因可能作为淋巴结转移的诊断标志物和治疗靶点。
