Adlerberth I, Svanborg C, Hanson L A, Carlsson B, Mellander L, Jalil F, Larsson P, Wold A E
Department of Clinical Immunology, University of Göteborg, Sweden.
FEMS Microbiol Lett. 1991 Nov 1;68(1):57-62. doi: 10.1016/0378-1097(91)90395-q.
The ability of Escherichia coli with different receptor specificities to interact with meconium was studied. E. coli strains expressing P-fimbriae, specific for Gal alpha 1-4Gal beta-containing receptors, were agglutinated by meconium at high titres. This reaction was inhibited by globotetraosylceramide. The attachment of P-fimbriated E. coli to human colonic epithelial cells of the HT-29 cell line was inhibited by meconium. Some type 1 fimbriated strains were agglutinated by meconium, but the agglutination was rarely blocked by methyl alpha-D-mannoside. The attachment by type 1 fimbriated strains to HT-29 cells was reduced by meconium only in some cases. These results suggest that meconium interacts with the P-fimbriae of E. coli, in a way that may influence bacterial colonization of the neonatal intestine.
研究了具有不同受体特异性的大肠杆菌与胎粪相互作用的能力。表达对含Galα1-4Galβ受体具有特异性的P菌毛的大肠杆菌菌株，被胎粪高滴度凝集。该反应被球四糖神经酰胺抑制。胎粪抑制了P菌毛化大肠杆菌与HT-29细胞系人结肠上皮细胞的附着。一些1型菌毛化菌株被胎粪凝集，但凝集很少被α-D-甲基甘露糖苷阻断。仅在某些情况下，胎粪减少了1型菌毛化菌株与HT-29细胞的附着。这些结果表明，胎粪与大肠杆菌的P菌毛相互作用，其方式可能影响新生儿肠道的细菌定植。
