Johanson I M, Plos K, Marklund B I, Svanborg C
Department of Medical Microbiology, Lund University, Sweden.
Microb Pathog. 1993 Aug;15(2):121-9. doi: 10.1006/mpat.1993.1062.
The pap gene clusters encode P fimbriae and fimbriae-associated G adhesins. DNA sequence analysis has resolved three G adhesin variants (papGJ96, papGIA2 and prsGJ96) that differ in receptor specificity and therefore in binding to epithelial cells. In this study, DNA probes specific for the pap gene cluster or the papGJ96, papGIA2 and prsGJ96 adhesin sequences were used to examine 74 fecal and 204 urinary Escherichia coli isolates (67 from acute pyelonephritis, 71 from acute cystitis and 66 from asymptomatic bacteriuria). In accordance with previous studies, a higher frequency of pap+ strains was found in the urinary strains (71%) than in the fecal (20%) E. coli isolates. The papGIA2, and prsGJ96 sequences were more frequent among urinary (42% papG+IA2, 23% prsG+J96) than among fecal (18% papG+IA2, 5% prsG+J96) isolates. None of the isolates hybridized with the papGJ96 probe. Pap+ strains accounted for 82% of the pyelonephritis, 69% of the cystitis and 61% of the asymptomatic bacteriuria strains. The papGIA2 genotype dominated in acute pyelonephritis strains (72% papG+IA2, 16% prsG+J96). The prsGJ96 genotype was most frequent in cystitis strains (25% papG+IA2, 37% prsG+J96). The asymptomatic bacteriuria strains formed an intermediate group (30% papG+IA2, 14% prsG+J96). Most of the papG+IA2 strains expressed P fimbriae which agglutinated human erythrocytes, sheep erythrocytes and Gal alpha 1-4Gal beta latex beads. The prsG+J96 strains varied in agglutination of human and sheep erythrocytes and Gal alpha 1-4Gal beta-latex beads. The results demonstrated that the papGIA2 and prsGJ96 adhesin DNA sequences differ in disease association.
pap基因簇编码P菌毛和与菌毛相关的G黏附素。DNA序列分析已解析出三种G黏附素变体（papGJ96、papGIA2和prsGJ96），它们在受体特异性上存在差异，因此在与上皮细胞的结合方面也有所不同。在本研究中，使用针对pap基因簇或papGJ96、papGIA2和prsGJ96黏附素序列的DNA探针，对74株粪便大肠杆菌分离株和204株尿液大肠杆菌分离株（67株来自急性肾盂肾炎，71株来自急性膀胱炎，66株来自无症状菌尿）进行检测。与先前的研究一致，在尿液分离株（71%）中发现的pap+菌株频率高于粪便大肠杆菌分离株（20%）。papGIA2和prsGJ96序列在尿液分离株（42% papG+IA2，23% prsG+J96）中比在粪便分离株（18% papG+IA2，5% prsG+J96）中更为常见。没有分离株与papGJ96探针杂交。Pap+菌株在肾盂肾炎菌株中占82%，在膀胱炎菌株中占69%，在无症状菌尿菌株中占61%。papGIA2基因型在急性肾盂肾炎菌株中占主导地位（72% papG+IA2，16% prsG+J96）。prsGJ96基因型在膀胱炎菌株中最为常见（25% papG+IA2，37% prsG+J96）。无症状菌尿菌株形成一个中间组（30% papG+IA2，14% prsG+J96）。大多数papG+IA2菌株表达能凝集人红细胞、绵羊红细胞和Galα1-4Galβ乳胶珠的P菌毛。prsG+J96菌株在人红细胞、绵羊红细胞和Galα1-4Galβ乳胶珠的凝集方面存在差异。结果表明，papGIA2和prsGJ96黏附素DNA序列在疾病关联方面存在差异。
