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评估纳米级和微米级磁性颗粒作为磁共振成像（MRI）细胞追踪造影剂的功效。

Assessing the efficacy of nano- and micro-sized magnetic particles as contrast agents for MRI cell tracking.

作者信息

Taylor Arthur, Herrmann Anne, Moss Diana, Sée Violaine, Davies Karen, Williams Steve R, Murray Patricia

机构信息

Institute of Translational Medicine, University of Liverpool, Liverpool, United Kingdom.

Institute of Integrative Biology, University of Liverpool, Liverpool, United Kingdom.

出版信息

PLoS One. 2014 Jun 24;9(6):e100259. doi: 10.1371/journal.pone.0100259. eCollection 2014.

DOI:10.1371/journal.pone.0100259
PMID:24959883
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4069012/
Abstract

Iron-oxide based contrast agents play an important role in magnetic resonance imaging (MRI) of labelled cells in vivo. Currently, a wide range of such contrast agents is available with sizes varying from several nanometers up to a few micrometers and consisting of single or multiple magnetic cores. Here, we evaluate the effectiveness of these different particles for labelling and imaging stem cells, using a mouse mesenchymal stem cell line to investigate intracellular uptake, retention and processing of nano- and microsized contrast agents. The effect of intracellular confinement on transverse relaxivity was measured by MRI at 7 T and in compliance with the principles of the '3Rs', the suitability of the contrast agents for MR-based cell tracking in vivo was tested using a chick embryo model. We show that for all particles tested, relaxivity was markedly reduced following cellular internalisation, indicating that contrast agent relaxivity in colloidal suspension does not accurately predict performance in MR-based cell tracking studies. Using a bimodal imaging approach comprising fluorescence and MRI, we demonstrate that labelled MSC remain viable following in vivo transplantation and can be tracked effectively using MRI. Importantly, our data suggest that larger particles might confer advantages for longer-term imaging.

摘要

基于氧化铁的造影剂在体内标记细胞的磁共振成像（MRI）中发挥着重要作用。目前，有多种此类造影剂可供使用，其尺寸从几纳米到几微米不等，由单个或多个磁芯组成。在此，我们使用小鼠间充质干细胞系来研究纳米级和微米级造影剂的细胞内摄取、保留和处理情况，评估这些不同颗粒对干细胞标记和成像的有效性。通过7T的MRI测量细胞内限制对横向弛豫率的影响，并按照“3R”原则，使用鸡胚模型测试造影剂在体内基于MR的细胞追踪中的适用性。我们表明，对于所有测试颗粒，细胞内化后弛豫率显著降低，这表明胶体悬浮液中造影剂的弛豫率不能准确预测基于MR的细胞追踪研究中的性能。使用包含荧光和MRI的双模态成像方法，我们证明标记的间充质干细胞在体内移植后仍保持活力，并且可以通过MRI有效地进行追踪。重要的是，我们的数据表明较大的颗粒可能在长期成像方面具有优势。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfb1/4069012/cdb7d34885fe/pone.0100259.g008.jpg
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