Zdarta Jakub, Klapiszewski Łukasz, Wysokowski Marcin, Norman Małgorzata, Kołodziejczak-Radzimska Agnieszka, Moszyński Dariusz, Ehrlich Hermann, Maciejewski Hieronim, Stelling Allison L, Jesionowski Teofil
Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, 60965 Poznan, Poland.
Institute of Inorganic Chemical Technology and Environmental Engineering, West Pomeranian University of Technology, Pulaskiego 10, 70322 Szczecin, Poland.
Mar Drugs. 2015 Apr 20;13(4):2424-46. doi: 10.3390/md13042424.
Innovative materials were made via the combination of chitin and lignin, and the immobilization of lipase from Aspergillus niger. Analysis by techniques including FTIR, XPS and 13C CP MAS NMR confirmed the effective immobilization of the enzyme on the surface of the composite support. The electrokinetic properties of the resulting systems were also determined. Results obtained from elemental analysis and by the Bradford method enabled the determination of optimum parameters for the immobilization process. Based on the hydrolysis reaction of para-nitrophenyl palmitate, a determination was made of the catalytic activity, thermal and pH stability, and reusability. The systems with immobilized enzymes were found to have a hydrolytic activity of 5.72 mU, and increased thermal and pH stability compared with the native lipase. The products were also shown to retain approximately 80% of their initial catalytic activity, even after 20 reaction cycles. The immobilization process, using a cheap, non-toxic matrix of natural origin, leads to systems with potential applications in wastewater remediation processes and in biosensors.
通过几丁质与木质素的结合以及黑曲霉脂肪酶的固定化制备了创新材料。包括傅里叶变换红外光谱（FTIR）、X射线光电子能谱（XPS）和13C交叉极化魔角旋转核磁共振（13C CP MAS NMR）在内的技术分析证实了酶在复合载体表面的有效固定化。还测定了所得体系的电动性质。元素分析和Bradford法得到的结果有助于确定固定化过程的最佳参数。基于对硝基苯基棕榈酸酯的水解反应，测定了催化活性、热稳定性和pH稳定性以及可重复使用性。发现固定化酶的体系具有5.72 mU的水解活性，与天然脂肪酶相比，热稳定性和pH稳定性有所提高。即使经过20个反应循环，产物仍显示保留其初始催化活性的约80%。使用廉价、无毒的天然来源基质的固定化过程导致所得体系在废水修复过程和生物传感器中具有潜在应用。
