Bagatolli Luis A
MEMPHYS - Center for Biomembrane Physics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense M, Denmark.
Biochim Biophys Acta. 2006 Oct;1758(10):1541-56. doi: 10.1016/j.bbamem.2006.05.019. Epub 2006 Jun 2.
In the last few years several experimental strategies based on epi-, confocal and two photon excitation fluorescence microscopy techniques have been employed to study the lateral structure of membranes using giant vesicles as model systems. This review article discusses the methodological aspects of the aforementioned experimental approaches, particularly stressing the information obtained by the use of UV excited fluorescent probes using two-photon excitation fluorescence microscopy. Additionally, the advantages of utilizing visual information, to correlate the lateral structure of compositionally simple membranes with complex situations, i.e., biological membranes, will be addressed.
在过去几年中，基于落射荧光、共聚焦和双光子激发荧光显微镜技术的几种实验策略已被用于以巨型囊泡作为模型系统来研究膜的横向结构。这篇综述文章讨论了上述实验方法的方法学方面，特别强调了使用双光子激发荧光显微镜通过紫外激发荧光探针获得的信息。此外，还将探讨利用视觉信息将组成简单的膜的横向结构与复杂情况（即生物膜）相关联的优势。
