Zimmern D
Nucleic Acids Res. 1975 Jul;2(7):1189-201. doi: 10.1093/nar/2.7.1189.
RNA extracted from CsC1-purified virions of tobacco mosaic virus is shown to give rise to an unusual nucleotide on digestion which RNAase T2, in addition to the four major nucleotides. This minor component has the electrophoretic characteristics of a phosphorylated end group, but is partially resistant to bacterial alkaline phosphatase. It is, however, a substrate for venom phosphodiesterase or nucleotide pyrophosphatase, yielding products which imply the structure m7G5'ppp5'Gp. TMV RNA, like many animal cellular and viral mRNAs recently examined, therefore has a 5' terminus blocked by a methylated nucleotide inverted with respect to the rest of the chain.
从烟草花叶病毒经氯化铯纯化的病毒粒子中提取的RNA，在消化时除了产生四种主要核苷酸外，还会产生一种不寻常的核苷酸。这种次要成分具有磷酸化末端基团的电泳特征，但对细菌碱性磷酸酶有部分抗性。然而，它是蛇毒磷酸二酯酶或核苷酸焦磷酸酶的底物，产生的产物表明其结构为m7G5'ppp5'Gp。因此，烟草花叶病毒RNA与最近研究的许多动物细胞和病毒mRNA一样，其5'末端被一个相对于链的其余部分反向的甲基化核苷酸所封闭。
