Ding Shaoxiong, Zhuang Xuan, Guo Feng, Wang Jun, Su Yongquan, Zhang Qiyong, Li Qifu
Department of Oceanography and Institute of Subtropical Oceanography, Xiamen University, Xiamen 361005, China.
Sci China C Life Sci. 2006 Jun;49(3):235-42. doi: 10.1007/s11427-006-0235-y.
The classification and evolutionary relationships are important issues in the study of the groupers. Cytochrome b gene fragment of twenty-eight grouper species within six genera of subfamily Epinephelinae was amplified using PCR techniques and the sequences were analyzed to derive the phylogenetic relationships of the groupers from the China Seas. Genetic information indexes, including Kimura-2 parameter genetic distance and Ts/Tv ratios, were generated by using a variety of biology softwares. With Niphon spinosus, Pagrus major and Pagrus auriga as the designated outgroups, phylogenetic trees, which invoke additional homologous sequences of other Epinephelus fishes from GenBank, were constructed based on the neighbor-joining (NJ), maximum-parsimony (MP), maximum-likelihood (ML) and minimum-evolution (ME) methods. Several conclusions were drawn from the DNA sequences analysis: (1) genus Plectropomus, which was early diverged, is the most primitive group in the subfamily Epinephelinae; (2) genus Variola is more closely related to genus Cephalopolis than the other four genera; (3) genus Cephalopolis is a monophyletic group and more primitive than genus Epinephelus; (4) Promicrops lanceolatus and Cromileptes altivelis should be included in genus Epinephelus; (5) there exist two sister groups in genus Epinephelus.
分类和进化关系是石斑鱼研究中的重要问题。利用PCR技术扩增了石斑鱼亚科6个属的28种石斑鱼的细胞色素b基因片段，并对序列进行分析，以推导中国海石斑鱼的系统发育关系。使用多种生物学软件生成了遗传信息指标，包括Kimura-2参数遗传距离和转换/颠换比率。以日本刺鲳、真鲷和黑棘鲷作为指定外类群，基于邻接法（NJ）、最大简约法（MP）、最大似然法（ML）和最小进化法（ME）构建了系统发育树，这些树引用了来自GenBank的其他石斑鱼的额外同源序列。从DNA序列分析中得出了几个结论：（1）早期分化的鳃棘鲈属是石斑鱼亚科中最原始的类群；（2）波纹唇鱼属与头鲈属比其他四个属的关系更密切；（3）头鲈属是一个单系群，比石斑鱼属更原始；（4）尖吻鲈和驼背鲈应归入石斑鱼属；（5）石斑鱼属存在两个姐妹群。
