Polzonetti V, Pucciarelli S, Signorello L, Vita A, Vincenzetti S, Natalini P
Dipartimento di Scienze Morfologiche e Biochimiche Comparate, University di Camerino, Italy.
Int J Immunopathol Pharmacol. 2004 Sep-Dec;17(3 Suppl):1-6.
In the present work we have quantified some enzymatic activities, related to NAD+ metabolism, in lymphocytes of healthy donors and of patients affected by B-cell chronic lymphocytic leukemia (B-CLL): NADase activity for NAD+ degradation and NMN adenylyltransferase (NMNAT) activity for NAD+ biosynthesis. Most of the samples under investigation (12 B-CLL patients and 12 healthy donors) presented the enzymatic activities assayed. No significant differences in terms of specific activity have been found between ill and healthy subjects. Nevertheless by expressing the activity as mU/10(9) cells (nmol min(-1) 10(9) cells) we observed significantly higher values of both enzymatic activities, involved in the NAD+ metabolism, in healthy donors with respect to the B-CLL patients.
在本研究中，我们对健康供体和B细胞慢性淋巴细胞白血病（B-CLL）患者淋巴细胞中一些与NAD+代谢相关的酶活性进行了定量：用于NAD+降解的NAD酶活性和用于NAD+生物合成的NMN腺苷酸转移酶（NMNAT）活性。大多数被研究的样本（12例B-CLL患者和12名健康供体）呈现出所检测的酶活性。在患病和健康受试者之间未发现比活性方面的显著差异。然而，通过将活性表示为mU/10⁹细胞（nmol min⁻¹ 10⁹细胞），我们观察到，与B-CLL患者相比，健康供体中参与NAD+代谢的两种酶活性值均显著更高。
