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简单鉴定携带单个整合基因拷贝的转基因拟南芥植株。

Simple identification of transgenic Arabidopsis plants carrying a single copy of the integrated gene.

作者信息

Kihara Tomonori, Zhao Cheng-Ri, Kobayashi Yuriko, Takita Eiji, Kawazu Tetsu, Koyama Hiroyuki

机构信息

Forestry Research Institute, Oji Paper, Kameyama, Mie 519-0212, Japan.

出版信息

Biosci Biotechnol Biochem. 2006 Jul;70(7):1780-3. doi: 10.1271/bbb.50687.

DOI:10.1271/bbb.50687
PMID:16861815
Abstract

Transgenic Arabidopsis thaliana plants carrying a single copy of integrated DNA can be identified by single-step genomic polymerase chain reaction. The reaction employs two sets of primer pairs with the same melting temperature that amplify the amplicons derived from the integrated T-DNA together with those from an endogenous single-copy gene as a reference. When the band intensity ratio is one, this means that the transgenic plants are carrying a single copy of the integrated gene per haploid.

摘要

携带单拷贝整合DNA的转基因拟南芥植株可通过单步基因组聚合酶链反应来鉴定。该反应使用两组具有相同解链温度的引物对，它们扩增来自整合T-DNA的扩增子以及来自内源性单拷贝基因的扩增子作为参照。当条带强度比为1时，这意味着转基因植株每个单倍体携带一个单拷贝的整合基因。

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