Federal Scientific Center of the East Asia Terrestrial Biodiversity of the Russian Academy of Sciences Far Eastern Branch, FGBUN FNC Bioraznoobrazia Nazemnoj Bioty Vostocnoj Azii Dal'nevostocnogo Otdelenia Rossijskoj Akademii Nauk, Vladivostok, 690022, Russia.
A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 690041, Russia.
Planta. 2022 Jun 11;256(1):8. doi: 10.1007/s00425-022-03927-x.
Increased flavonol accumulation and enhanced drought tolerance in A4-rolB-overexpressing plants can be explained by the cooperative action of the SA and ROS signalling pathways. Clarification of function of the A4-rolB plast gene from pRiA4 of Rhizobium rhizogenes will allow a better understanding of the biological principles of the natural transformation process and its use as a tool for plant bioengineering. In the present study, we investigated whether the overexpression of A4-rolB gene could regulate two important processes, flavonoid biosynthesis and drought tolerance. In addition, we investigated some aspects of the possible machinery of the A4-rolB-induced changes in plant physiology, such as crosstalk of the major signalling systems. Based on the data obtained in this work, it can be presumed that constitutive overexpression of A4-rolB leads to the activation of the salicylic acid signalling system. An increase in flavonol accumulation and enhanced drought tolerance can be explained by the cooperative action of SA and ROS pathways.
在 A4-rolB 过表达植物中，类黄酮积累增加和耐旱性增强可以用 SA 和 ROS 信号通路的协同作用来解释。阐明根瘤农杆菌 pRiA4 中 A4-rolB 质体基因的功能将有助于更好地理解自然转化过程的生物学原理及其作为植物生物工程工具的应用。在本研究中，我们研究了 A4-rolB 基因的过表达是否可以调节两个重要过程，类黄酮生物合成和耐旱性。此外，我们还研究了 A4-rolB 诱导的植物生理学变化的可能机制的一些方面，例如主要信号系统的串扰。基于本工作获得的数据，可以推测 A4-rolB 的组成型过表达导致水杨酸信号系统的激活。类黄酮积累增加和耐旱性增强可以用 SA 和 ROS 途径的协同作用来解释。
