Yip V, Carter J G, Pusateri M E, McDougal D B, Lowry O H
Department of Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110.
Neurochem Res. 1991 Jun;16(6):629-35. doi: 10.1007/BF00965548.
Eleven regions of mouse brain and twelve layers of monkey retina were assayed for choline acetyl transferase (ChAT), acetylcholine esterase (AChE), and 4 enzymes that synthesize acetyl CoA. The purpose was to seek evidence concerning the source of acetyl CoA for acetylcholine generation. In brain ATP citrate lyase was strongly correlated with ChAT as well as AChE (r = 0.914 in both cases). Weak, but statistically significant correlation, was observed between ChAT and both cytoplasmic and mitochondrial thiolase, whereas there was a significant negative correlation between ChAT and acetyl thiokinase. In retina ChAT was essentially limited to the inner plexiform and ganglion cell layers, whereas substantial AChE activity extended as well into inner nuclear, outer plexiform and fiber layers, but no further. ATP citrate lyase activity was also highest in the inner four retinal layers, but was not strongly correlated with either ChAT or AChE (r = 0.724 and 0.761, respectively). Correlation between ChAT and acetyl thiokinase was at least as strong (r = 0.757), and in the six inner layers of retina, the correlation between ChAT and acetylthiokinase was very strong (r = 0.932).
对小鼠大脑的11个区域和猴子视网膜的12层进行了胆碱乙酰转移酶（ChAT）、乙酰胆碱酯酶（AChE）以及4种合成乙酰辅酶A的酶的检测。目的是寻找有关乙酰胆碱生成中乙酰辅酶A来源的证据。在大脑中，ATP柠檬酸裂解酶与ChAT以及AChE都高度相关（两种情况的r均为0.914）。ChAT与细胞质和线粒体硫解酶之间观察到较弱但具有统计学意义的相关性，而ChAT与乙酰硫激酶之间存在显著的负相关。在视网膜中，ChAT主要局限于内网状层和神经节细胞层，而大量的AChE活性也延伸到内核层、外网状层和纤维层，但没有更远。ATP柠檬酸裂解酶活性在视网膜内四层中也最高，但与ChAT或AChE均无强相关性（分别为r = 0.724和0.761）。ChAT与乙酰硫激酶之间的相关性至少同样强（r = 0.757），并且在视网膜的六个内层中，ChAT与乙酰硫激酶之间的相关性非常强（r = 0.932）。
