Tsai Ju-Hsin, Hsu Chun-Sen, Tsai Chung-Hung, Su Jang-Ming, Liu Yin-Tso, Cheng Min-Hsiung, Wei James Cheng-Chung, Chen Fong-Lin, Yang Chi-Chiang
Department of Surgery, Chung Shan Medical University Hospital, Chung Shan Medical University, Taichung, Taiwan, ROC.
J Cancer Res Clin Oncol. 2007 Jan;133(1):13-21. doi: 10.1007/s00432-006-0141-5. Epub 2006 Jul 25.
Our previous study based on the results of polymerase chain reaction and Southern hybridization for the detection of Human papilloma virus (HPV), Cytomegalovirus (CMV), Epstein-Barr virus (EBV), Herpes simplex virus (HSV)-1, HSV-2, and Human herpesvirus (HHV)-8 DNA in non-familial breast cancer patients suggest that the viruses associated with breast cancer are HHV-8 > EBV (P < 0.01). Therefore, efforts were made to further investigate the association between breast cancer with nodal status and viral infections.
Sixty-two breast cancer patients and their mammary specimens were enrolled in this retrospective study. The presence of these six potential oncogenic viruses was analyzed to establish the relationship between nodal status and treatment outcome. Statistical analyses were used for the assessment of variables, including viral positivity and clinical feature.
Viral positivity was not significantly different comparing node-positive and node-negative patients (P > 0.05). When the viral factors were not entered for statistical analyses, no variable was significantly related to overall survival. However, tumor stage, tumor size, nodal status , and estrogen receptor were significantly related to relapse-free survival (P < 0.05). For viral factors, the number of infecting viruses is related to the overall and relapse-free survivals. Only when V0 or V(0, 1) was grouped for comparison with other multiply virus-infected subgroups, were the overall and relapse-free survivals significantly different (P < 0.005 or P < 0.001). The results suggest that HSV-1, HHV-8, EBV, CMV, and HPV were related to overall survival, however, only HHV-8 and CMV were related to relapse-free survival (P < 0.05 or P < 0.01).
Virus factor is significantly related to human breast cancer, not only in terms of the oncogenetic process, but also in overall and relapse-free survivals.
我们之前基于聚合酶链反应和Southern杂交结果对非家族性乳腺癌患者检测人乳头瘤病毒（HPV）、巨细胞病毒（CMV）、爱泼斯坦-巴尔病毒（EBV）、单纯疱疹病毒（HSV）-1、HSV-2和人类疱疹病毒（HHV）-8 DNA的研究表明，与乳腺癌相关的病毒是HHV-8 > EBV（P < 0.01）。因此，我们进一步努力研究乳腺癌与淋巴结状态及病毒感染之间的关联。
本回顾性研究纳入了62例乳腺癌患者及其乳腺标本。分析这六种潜在致癌病毒的存在情况，以确定淋巴结状态与治疗结果之间的关系。采用统计分析评估变量，包括病毒阳性情况和临床特征。
淋巴结阳性和阴性患者的病毒阳性率无显著差异（P > 0.05）。在未纳入病毒因素进行统计分析时，没有变量与总生存期显著相关。然而，肿瘤分期、肿瘤大小、淋巴结状态和雌激素受体与无复发生存期显著相关（P < 0.05）。对于病毒因素，感染病毒的数量与总生存期和无复发生存期相关。仅当将V0或V(0, 1)分组与其他多重病毒感染亚组进行比较时，总生存期和无复发生存期才有显著差异（P < 0.005或P < 0.001）。结果表明，HSV-1、HHV-8、EBV、CMV和HPV与总生存期相关，然而，只有HHV-8和CMV与无复发生存期相关（P < 0.05或P < 0.01）。
病毒因素不仅在致癌过程中，而且在总生存期和无复发生存期方面都与人类乳腺癌显著相关。
