Erdei Esther, Lee Sang-Joon, Wei Qingyi, Wang Li-E, Song Yan-S, Bovbjerg Dana, Berwick Marianne
University of New Mexico, School of Medicine, Department of Internal Medicine and New Mexico Cancer Research and Treatment Center, Albuquerque, NM 87131, USA.
Mutagenesis. 2006 Jul;21(4):261-4. doi: 10.1093/mutage/gel030. Epub 2006 Jul 26.
Mutagen sensitivity is regarded as a genetic susceptibility phenotype for various cancers; it is cytogenetically based and probably involves a number of genes from different DNA repair pathways. This assay has been used in a number of laboratories in the field of epidemiology, where it has been investigated and appears to be a useful susceptibility biomarker for epidemiological studies assessing cancer risks at the population level. One concern about phenotypic assays, such as the mutagen sensitivity assay, has been that there could be wide variation in results depending on the timing of the assay (within individual variation), the individual performing the assay (within observer variation) and the laboratory where the assay has been performed (inter-laboratory variation). We conducted an inter-laboratory comparison study between the Memorial Sloan-Kettering Cancer Center and M. D. Anderson, in which we assessed all these concerns. We did not find any significant variation in any of the assays. The correlation was high for all tests. The good concordance rate between laboratories supports the continued use of the mutagen sensitivity assay by different laboratories, and demonstrates its potential to identify at-risk subgroups among normal individuals and cancer patients alike.
诱变敏感性被视为多种癌症的一种遗传易感性表型；它基于细胞遗传学，可能涉及来自不同DNA修复途径的多个基因。该检测方法已在流行病学领域的多个实验室中使用，在这些实验室中它得到了研究，并且似乎是在人群水平评估癌症风险的流行病学研究中一种有用的易感性生物标志物。对于诸如诱变敏感性检测这样的表型检测方法，一个担忧是，根据检测时间（个体内部差异）、进行检测的个体（观察者内部差异）以及进行检测的实验室（实验室间差异），结果可能会有很大差异。我们在纪念斯隆凯特琳癌症中心和MD安德森癌症中心之间进行了一项实验室间比较研究，在该研究中我们评估了所有这些问题。我们在任何一项检测中均未发现任何显著差异。所有检测的相关性都很高。实验室之间良好的一致性率支持不同实验室继续使用诱变敏感性检测方法，并证明了其在识别正常个体和癌症患者中高危亚组的潜力。
