Li Chunying, Wang Li-E, Wei Qingyi
Department of Epidemiology, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, USA.
Int J Cancer. 2009 Mar 1;124(5):999-1007. doi: 10.1002/ijc.24126.
DNA repair is a complicated biological process, consisting of several distinct pathways, that plays a fundamental role in the maintenance of genomic integrity. The very important field of DNA repair and cancer risk has developed rapidly in the past decades. In this review of selected published data from our laboratory, we describe mostly our work on the study of phenotypic markers of nucleotide excision repair (NER), as measured by the benzo(a)pyrene diol epoxide (BPDE)/ultraviolet (UV)-induced mutagen sensitivity assays, BPDE-induced adduct assay, host cell reactivation (HCR)-DNA repair capacity (DRC) assay, reverse transcription-polymerase chain reaction (RT-PCR) assay and reverse-phase protein lysate microarray (RPP) assay, by using peripheral blood lymphocytes in a series of molecular epidemiological studies. Results of our studies suggest that individuals with reduced DRC have an elevated cancer risk. This finding needs additional validation by other investigators, and we also discussed issues in conducting this kind of research in the future.
DNA修复是一个复杂的生物学过程，由几个不同的途径组成，在维持基因组完整性方面发挥着基础性作用。在过去几十年里，DNA修复与癌症风险这个非常重要的领域发展迅速。在这篇对我们实验室已发表的选定数据的综述中，我们主要描述了我们在核苷酸切除修复（NER）表型标志物研究方面的工作，这些标志物通过苯并(a)芘二醇环氧化物（BPDE）/紫外线（UV）诱导的诱变敏感性测定、BPDE诱导的加合物测定、宿主细胞再激活（HCR）-DNA修复能力（DRC）测定、逆转录-聚合酶链反应（RT-PCR）测定以及反相蛋白质裂解物微阵列（RPP）测定来衡量，我们在一系列分子流行病学研究中使用了外周血淋巴细胞。我们的研究结果表明，DRC降低的个体患癌风险升高。这一发现需要其他研究者进一步验证，我们还讨论了未来开展这类研究时的相关问题。
