人乳头瘤病毒DNA暴露与胚胎存活具有阶段特异性。
Human papilloma virus DNA exposure and embryo survival is stage-specific.
作者信息
Henneberg Andrew A, Patton William C, Jacobson John D, Chan Philip J
机构信息
Department of Gynecology and Obstetrics, Loma Linda University School of Medicine, Loma Linda, California 92354, USA.
出版信息
J Assist Reprod Genet. 2006 Jun;23(6):255-9. doi: 10.1007/s10815-006-9030-8. Epub 2006 Jul 27.
PURPOSE
Human papillomavirus (HPV) has been shown to disrupt late-stage implanting embryos. The objectives were (a) to assess the development of early embryos exposed to HPV DNA and (b) to analyze the blastocyst hatching process after HPV exposure.
METHODS
The study involved exposing two-cell and 4-8-cell mouse embryos to DNA fragments from either HPV type 16, type 18 or DQA1 (control). The embryos were incubated for 120 h and assessed.
RESULTS
HPV 16 and 18 inhibited two-cell embryo development. In contrast, delaying the exposure of HPV DNA until the 4-8-cell stage resulted in further embryonic development. There was 25.9% less blastocyst formed with HPV 16 exposure. Additionally, there were 25.9-31.8% more degenerated embryos with HPV 16 exposure.
CONCLUSIONS
The study demonstrated embryo stage-specific effects of HPV on early development. The results suggested HPV exposure was linked to two-cell embryo demise and delaying the exposure of HPV until later embryo stages permitted embryo development. HPV 16 was shown to decrease blastocyst formation while HPV 18 inhibited the blastocyst hatching process.
目的
人乳头瘤病毒（HPV）已被证明会破坏晚期着床胚胎。本研究的目的是：（a）评估暴露于HPV DNA的早期胚胎的发育情况；（b）分析HPV暴露后囊胚孵化过程。
方法
本研究将二细胞期和4-8细胞期的小鼠胚胎暴露于16型HPV、18型HPV或DQA1（对照）的DNA片段中。将胚胎培养120小时后进行评估。
结果
16型和18型HPV抑制二细胞期胚胎发育。相反，将HPV DNA的暴露推迟到4-8细胞期会使胚胎进一步发育。暴露于16型HPV的胚胎形成的囊胚减少了25.9%。此外，暴露于16型HPV的退化胚胎增加了25.9%-31.8%。
结论
该研究证明了HPV对早期发育具有胚胎阶段特异性影响。结果表明，HPV暴露与二细胞期胚胎死亡有关，将HPV暴露推迟到胚胎后期可使胚胎发育。研究表明，16型HPV会减少囊胚形成，而18型HPV会抑制囊胚孵化过程。
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