Hermonat P L, Kechelava S, Lowery C L, Korourian S
Department of Obstetrics and Gynecology, University of Arkansas for Medical Sciences, Little Rock 72205, USA.
Hum Pathol. 1998 Feb;29(2):170-4. doi: 10.1016/s0046-8177(98)90228-3.
In a recent study it has been shown that human papillomavirus (HPV) infection was threefold more prevalent in spontaneous abortion specimens compared with elective specimens (60% [15 of 25] v 20% [3 of 15], respectively) as analyzed by broad-spectrum HPV polymerase chain reaction (PCR) amplification and dot-blot hybridization with an HPV-16 probe. In this study, archival paraffin-embedded tissue from a subset of previously analyzed spontaneous abortion cases were reanalyzed by in situ PCR amplification so that the specific cells infected by HPV could be identified. In the current study, using a new PCR primer set for HPV-16 E6, the status of six previously analyzed cases were verified (five HPV-positive and one negative). Furthermore, syncytiotrophoblasts were identified as the predominant cellular target of HPV (HPV-16 or a related type). Finally, four of four third-trimester placentas similarly analyzed gave no HPV-positive signal. Trophoblasts are the cell type that maintains placental contact with maternal tissue and through which nutrient exchange occurs. This knowledge prompts the hypothesis that HPV-infected trophoblasts may have altered characteristics, which may lead to a compromised gestation.
最近的一项研究表明，通过广谱人乳头瘤病毒（HPV）聚合酶链反应（PCR）扩增以及用HPV - 16探针进行斑点杂交分析，发现自然流产标本中HPV感染的发生率是选择性流产标本的三倍（分别为60%[25例中的15例]对20%[15例中的3例]）。在这项研究中，对之前分析过的一部分自然流产病例的存档石蜡包埋组织进行原位PCR扩增重新分析，以便能够识别出被HPV感染的特定细胞。在当前研究中，使用针对HPV - 16 E6的一组新的PCR引物，对之前分析过的6例病例的情况进行了验证（5例HPV阳性，1例阴性）。此外，合体滋养层细胞被确定为HPV（HPV - 16或相关类型）的主要细胞靶点。最后，对同样分析的4例孕晚期胎盘进行检测，均未给出HPV阳性信号。滋养层细胞是维持胎盘与母体组织接触并进行营养物质交换的细胞类型。这一认识促使人们提出假说，即被HPV感染的滋养层细胞可能具有改变的特性，这可能导致妊娠受损。
